USA flag logo/image

An Official Website of the United States Government

Exon Boundary Tags (EBTs) for Human Functional Genome Annotation

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
80236
Program Year/Program:
2006 / SBIR
Agency Tracking Number:
HG004180
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NEWLINK GENETICS CORPORATION
NEWLINK GENETICS CORPORATION 2901 S LOOP DR, BLDG 3, STE 3900 AMES, IA 50010
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2006
Title: Exon Boundary Tags (EBTs) for Human Functional Genome Annotation
Agency: HHS
Contract: 1R43HG004180-01
Award Amount: $98,414.00
 

Abstract:

DESCRIPTION (provided by applicant): The Human Genome Project has brought the field of functional genomics to the forefront of biological and pharmaceutical research. However, deciphering the function(s) of each gene in a high throughput format is complicated by alternative splicing and post- translational modifications of proteins. Serial Analysis of Vector Integration (SAVI(r)) is a patented method for high-throughput acquisition of genome-wide functional exons and gene expression profiling by sequencing only a certain length of each exon boundary as Exon Boundary Tag (EBT) after retroviral gene trapping. Since the EBTs generated will be proportioned to the copy numbers of corresponding mRNA molecules or alternative transcripts, quantification of acquired EBTs can reflect the transcription level for gene expression profiles between different treatments or cell types, such as cancer vs. normal cells, for pharmaceutical developments. Preliminary studies demonstrate the feasibility of generating unique data in this research field with a high potential for functional genome annotation, cDNA collection and exon/splice-specific microarray design. The study we are proposing here is a high-throughput acquisition of genome- wide functional exons by sequencing only a certain length of each exon boundary to assemble open reading frames for functional genome annotation and gene expression profiling.

Principal Investigator:

Wonbin Young
5152963530
wyoung@linkp.com

Business Contact:


5152963530
sjohnson@linkp.com
Small Business Information at Submission:

NEWLINK GENETICS CORPORATION
NEWLINK GENETICS CORPORATION 2901 S LOOP DR, BLDG 3, STE 3900 AMES, IA 50010

EIN/Tax ID: 421491350
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No