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Fluorescent Polymer Nanoparticle Gene Expression System

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66525
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
GM067507
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Nomadics, Inc.
1024 S. Innovation Way Stillwater, OK 74074
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Fluorescent Polymer Nanoparticle Gene Expression System
Agency: HHS
Contract: 1R43GM067507-01
Award Amount: $400,001.00
 

Abstract:

DESCRIPTION (provided by applicant): Nomadics, Inc. proposes to use amplifying fluorescent polymer (AFP) nanoparticles to improve the sensitivity of nucleic acid hybridization assays by up to 5 orders of magnitude. This will directly impact gene expression studies by improving sensitivity and enabling quantitative comparison with reference samples. Rapid energy migration of the excited state along the conjugated polymer backbone of fluorescent polymers amplifies the optical signature of a molecular quenching or FRET event. Amplification is further enhanced by three-dimensional energy migration in a condensed solid. Nomadics has developed materials and methods for the facile preparation of aqueous nanoparticle dispersions of fluorescent poly(phenylene ethynylene) polymers. This will enable application of these materials for the detection of nucleic acid hybridization in homogeneous assays and array formats. In this Phase I effort, Nomadics will expand our techniques for the fabrication of fluorescent polymer nanoparticles; define methods for preparing nanoparticle-nucleic acid conjugates; demonstrate transduction of the nanoparticte fluorescence via nucleic acid hybridization; and evaluate the sensitivity potential of nanoparticles in a microarray format. Phase II will develop quantitative microarray-based systems for gene expression studies that leverage the large optical signals generated by AFP nanoparticles.

Principal Investigator:

Lawrence F. Hancock
6174418871
LHANCOCK@NOMADICS.COM

Business Contact:

James Luby
4053729535
JLUBY@NOMADICS.COM
Small Business Information at Submission:

NOMADICS, INC.
NOMADICS, INC. 1024 S INNOVATION WAY STILLWATER, OK 74074

EIN/Tax ID: 731443947
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No