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Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk…
- Small Business Information
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Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
- Phase 1
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Fiscal Year: 2004Title: Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk Chronic Wasting DiseaseAgency / Branch: DOD / ARMYContract: W81XWH-04-C-0141Award Amount: $100,000.00
Abstract:
The goal of the proposed research is to develop a cell culture model for elk chronic wasting disease (CWD) prion propagation that can be used as a bioassay for detecting CWD. Our goal will be accomplished by bioengineering a bovine B-lymphocyte cell (B-cell) line to surface express elk PrPc fused to yellow fluorescent protein (YFP). Interaction of these B-cells with infectious, mis-folded, protease resistant CWD prion (PrPcwd) will induce conversion of the dispersed YFP-PrPc to aggregated mis-folded YFP-PrPcwd that will be detected by confocal microscopy as aggregated YFP-PrPcwd on the B-cell surface. Once Phase I proof-of-concept has been demonstrated by elk PrPcwd conversion of YFP-PrPc on the B-cell line to YFP-PrPcwd, a stably transfected cell line will be developed and validated as a sensitive, specific, and reproducible bioassay for detecting PrPcwd infected elk and deer tissues and body fluids during Phase II.Small Business Information at Submission:Research Institution Information:NOMADICS, INC.
1024 S. Innovation Way Stillwater, OK 74074EIN/Tax ID: 731443947DUNS: N/ANumber of Employees:Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: NoOklahoma State University
College of Veterinary Medicine, 221 McElroy Hall
Stillwater, OK 74078Contact: Kenneth ClinkenbeardContact Phone: (405) 744-4467RI Type: Nonprofit college or university
Award Information
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Agency: Department of Defense |
Branch: Army |
Award ID: 68463 |
Program Year/Program: 2004 / STTR |
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Agency Tracking Number: A045-027-0237 |
Solicitation Year: N/A |
Solicitation Topic Code: N/A |
Solicitation Number: N/A |