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Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk…

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
68463
Program Year/Program:
2004 / STTR
Agency Tracking Number:
A045-027-0237
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Nomadics, Inc.
1024 S. Innovation Way Stillwater, OK 74074
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk Chronic Wasting Disease
Agency / Branch: DOD / ARMY
Contract: W81XWH-04-C-0141
Award Amount: $100,000.00
 

Abstract:

The goal of the proposed research is to develop a cell culture model for elk chronic wasting disease (CWD) prion propagation that can be used as a bioassay for detecting CWD. Our goal will be accomplished by bioengineering a bovine B-lymphocyte cell (B-cell) line to surface express elk PrPc fused to yellow fluorescent protein (YFP). Interaction of these B-cells with infectious, mis-folded, protease resistant CWD prion (PrPcwd) will induce conversion of the dispersed YFP-PrPc to aggregated mis-folded YFP-PrPcwd that will be detected by confocal microscopy as aggregated YFP-PrPcwd on the B-cell surface. Once Phase I proof-of-concept has been demonstrated by elk PrPcwd conversion of YFP-PrPc on the B-cell line to YFP-PrPcwd, a stably transfected cell line will be developed and validated as a sensitive, specific, and reproducible bioassay for detecting PrPcwd infected elk and deer tissues and body fluids during Phase II.

Principal Investigator:

David Goad
Principal Investigator
4053729535
dgoad@nomadics.com

Business Contact:

James H. Luby
Chief Operating Officer
4053729535
jluby@nomadics.com
Small Business Information at Submission:

NOMADICS, INC.
1024 S. Innovation Way Stillwater, OK 74074

EIN/Tax ID: 731443947
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
Oklahoma State University
College of Veterinary Medicine, 221 McElroy Hall
Stillwater, OK 74078
Contact: Kenneth Clinkenbeard
Contact Phone: (405) 744-4467
RI Type: Nonprofit college or university