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Fluorescence-based Multiplexed Universal Virus Detection Platform

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
74028
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
A052-165-0477
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Nomadics, Inc.
1024 S. Innovation Way Stillwater, OK 74074
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: Fluorescence-based Multiplexed Universal Virus Detection Platform
Agency / Branch: DOD / ARMY
Contract: W81XWH-06-C-0048
Award Amount: $69,999.00
 

Abstract:

The aim of this study is to develop a multiplexed virus detection platform capable of detecting within three hours the presence of any known virus, genetically modified virus or unidentified virus, in clinical samples like serum. A PCR based technique will be employed to identify the presence of unique viral sequences. In its ultimate form, the proposed system will encompass a simple and rapid sample preparation step followed by PCR, fluorophore tagging (using a highly stable and novel amplifying fluorescent polymer), and hybridization to a virus-specific oligonucleotide microarray printed on the cap of a PCR tube. The system will also allow for the cloning and subsequent sequencing of nucleic acids to establish the presence of unknown viruses. This diagnostic platform will find applications in screening banked blood for the presence of viruses, detecting the presence of unnatural (mutated) viruses used as bio-warfare agents, and detecting the presence of latent viruses in the human system. In Phase 1 we will perform the following objectives: 1) Standardize and optimize the PCR, 2) Test and optimize the sample preparation protocol, 3) Fabricate microarrays, 4) Optimize microarray hybridization, 5) Clone and sequence PCR products, and 6) Evaluate the sensitivity and specificity of the assay.

Principal Investigator:

Akhilesh Ramachandran
Principal Investigator
4053729535
aramachandran@nomadics.com

Business Contact:

Jim Luby
COO
4053729535
jluby@nomadics.com
Small Business Information at Submission:

NOMADICS, INC.
1024 S. Innovation Way Stillwater, OK 74074

EIN/Tax ID: 731443947
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No