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ERYTHROPOIETIN (EP) IS A PRIMARY REGULATOR OF ERYTHROPOIESIS.

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
1378 		Program Year/Program:
1984 / SBIR
Agency Tracking Number:
1378 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
North Valley Farms Inc.
3 Bancroft Park Hopedale, MA 01747
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1984
Title: ERYTHROPOIETIN (EP) IS A PRIMARY REGULATOR OF ERYTHROPOIESIS.
Agency: HHS
Contract: N/A
Award Amount: $49,453.00
 

Abstract:

ERYTHROPOIETIN (EP) IS A PRIMARY REGULATOR OF ERYTHROPOIESIS. ACCORDINGLY, ITS POSSIBLE DIAGNOSTIC AND TREATMENT APPLICATIONS IN CERTAIN REFRACTORY ANEMIAS HAS BEEN POSTULATED. THE LACK OF BOTH RAW MATERIAL FOR ERYTHROPOIETIN PURIFICATION AND RELATIVELY PURE EP (>150 UNITS/MG PROTEIN) HAS SEVERELY COMPROMISED ITS USE IN RESEARCH, DIAGNOSIS, AND TREATMENT. FURTHERMORE, THIS SITUATION HAS BEEN COMPLICATED BY THE LACK OF AN ACCURATE, SIMPLE ASSAY FOR BIOLOGICALLY ACTIVE EP. ACCORDINGLY, THE LONG TERM OBJECTIVES OF THIS INVESTIGATION ARE THE DEVELOPMENT OF ECONOMICAL PROCEDURES FOR THE GENERATION, ASSAY AND PURIFICATION OF ERYTHROPOIETIN. THROUGH THE USE OF SHEEP ALONG WITH A NOVEL BLEED-PLASMAPHERESIS PROCEDURE, IT IS BELIEVED THAT LARGE VOLUMES OF EP-RICH PLASMA (2-10 UNITS/ML) CAN BE ECONOMICALLY PRODUCED. THIS APPROACH WILL ALLOW FOR A LOW COST SUPPLY OF PLASMA FOR EP ASSAY AND PURIFICATION. THE IN VITRO PLASMA CLOT TECHNIC FOR CULTURING ERYTHROID PROGENITORS WILL BE REFINED AND DEVELOPED AS AN ERYTHROPOIETIN BIOASSAY. PURIFICATION WILL INITIALLY RELY ON A SIMPLIFIED PARTIAL PURIFICATION PROCEDURE BASED ON NITROCELLULOSE-EP BINDING AFFINITY AND STANDARD PURIFICATION PROCEDURES TO YIELD A PURE ENOUGH EP ANTIGEN TO GENERATE HIGHLY SPECIFIC EP-ANTISERA (POLYCLONAL AND/OR MONOCLONAL) WHICH COULD IN TURN BE USED TO EFFICIENTLY PURIFY ERYTHROPOIETIN.

Principal Investigator:

Dr. peter p. paletra
0

Business Contact:

Small Business Information at Submission:

North Valley Farms Inc.
3 Bancroft Park Hopedale, MA 01747

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No