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PRACTICAL SYNTHESIS OF PHOSPHOINOSITIDES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4945
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
4945
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NUTRIMED BIOTECH
CORNELL UNIVERSITY RESEARCH PARK 270-276 LANGMUIR LAB, BROWN RD ITHACA, NY 14850
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1989
Title: PRACTICAL SYNTHESIS OF PHOSPHOINOSITIDES
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

THERE IS A CURRENT AND GROWING INTEREST IN THE ROLE OF MEMBRANE PHOSPHOINOSITIDES IN CELL BIOLOGY, ESPECIALLY IN RELATION TO THE TRANSMISSION OF CA2+ MEDIATED SIGNALS. THESE SIGNALS ARE GENERATED WHEN CELL-SURFACE RECEPTORS ARE ACTIVATED BY HORMONES, NEUROTRANSMITTERS, GROWTH FACTORS, AND OTHER AGONISTS, IN RELATION TO THE MECHANISM OF MALIGNANT TRANSFORMATIONS INDUCED BY ONCOGENES AND BY THE ACTIVATION OF KINASE C. SAMPLES OF PURE PHOSPHOINOSITIDES AND ANALOGS ARE IMPORTANT FOR RESEARCH AND AS POTENTIAL THERAPEUTIC AGENTS. PRACTICAL METHODS FOR THEIR PREPARATIONBY SYNTHESIS ARE NOT AVAILABLE, HOWEVER. THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A GENERAL, SIMPLE, AND PRACTICAL METHOD FOR THE SYNTHESIS OF DISTINCT MOLECULARSPECIES OF PHOSPHATIDYLINOSITOL (PI), PHOSPHATIDYLINOSITOL- 4-PHOSPHATE (PIP), AND PHOSPHATIDYLINOSITOL-4,5- BISPHOSPHATE (PIP2), EACH CARRYING FATTYACYL RESIDUES OF CHOICE IN POSITIONS 1',2'. THE MIXTURES OF FATTYACYLS IN NATURAL PI AND DERIVATIVES WITH ANY DESIRED FATTYACYLS WILL BE REPLACED BY A DEACYLATION-REACYLATION CYCLE, OR BY REMOVAL AND REINTRODUCTION OF THE COMPLETE DIGLYCERIDE RESIDUE. THE SYNTHESIS DEPENDS ON AN INNOVATIVE APPLICATIONOF LIPASES IN A COMPOSITE CHEMICAL AND ENZYMATIC SEQUENCE. DURING PHASE I, THIS ASPECT AND THE PREREQUISITE PROTECTION AND DEBLOCKING OF THE INOSITOL HYDROXYLS WILL BE EVALUATED FOR THE PREPARATION OF SEMISYNTHETIC 1',2'-DIACYL-SN- GLYCEROPHOSPHOINOSITOLS FROM PI ISOLATED FROM SOY-LECITHIN, TO DETERMINE THE FEASIBILITY OF THE GENERAL STRATEGY.

Principal Investigator:

Dr Rajindra Aneja
Principal Investigator
6072571166

Business Contact:

Small Business Information at Submission:

Nutrimed Biotech
270-276 Langmuir Laboratory Brown Road Ithaca, NY 14850

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No