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IMPROVED METHOD FOR ADENOVIRAL VECTORS CONSTRUCTION

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
54517
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
1R43GM062043-01
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
O.D. 260, Inc.
624 N. Hillview Dr Boise, ID 83712
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: IMPROVED METHOD FOR ADENOVIRAL VECTORS CONSTRUCTION
Agency: HHS
Contract: N/A
Award Amount: $129,946.00
 

Abstract:

DESCRIPTION (adapted from applicant's abstract): An innovative system is proposed for the utilization of the adenoviral terminal protein to facilitate the construction of adenoviral vectors used for gene transfer, and in particular gene therapy. So far, adenoviral vectors are best generated using bacterial plasmids, because they eliminate potential viral contaminations. However, plasmid-derived adenoviral DNAs are poorly infectious, and the recovery of the virus sometimes can take several weeks. There is ample evidence in literature, and our preliminary data have confirmed, that the presence of the terminal protein at both ends of the adenoviral DNA increases its infectivity by two or three orders of magnitude. Our goal is to increase the infectivity of adenoviral DNAs prepared from plasmids, by using the terminal protein. We propose 1) a method for purifying the terminal protein and, b) a method to bind the purified protein to the ends of adenoviral DNAs derived from the plasmids. We brought together a strong team and the resources necessary to demonstrate our concept. We expect this development will provide a more expedient and thus less expensive method for the construction of first-generation, second-generation and gutless adenoviral vectors, than currently available. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

Principal Investigator:

Xavier C. Danthinne

Business Contact:


2083361686
XDANTHIN@MICRON.NET
Small Business Information at Submission:

O.D. 260, INC.
624 N HILLVIEW DR BOISE, ID 83713

EIN/Tax ID: 820520470
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No