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CELL CULTURE IN MICROGRAVITY: AN INSTRUMENT TO MONITOR GROWTH AND CELL MARKERS

Award Information

Agency:
National Aeronautics and Space Administration
Branch:
N/A
Award ID:
6763
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
6763
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
OPTRA, Inc
461 Boston Street Topsfield, MA 01983-1290
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: CELL CULTURE IN MICROGRAVITY: AN INSTRUMENT TO MONITOR GROWTH AND CELL MARKERS
Agency: NASA
Contract: N/A
Award Amount: $49,465.00
 

Abstract:

EXISTING METHODS IN CELL CULTURE FOR MONITORING POPULATION GROWTH AND FOR LOCALIZING AND QUANTIFYING CELLULAR BIOMOLECULES REQUIRE HARVESTING OF CELLS, EXTENSIVE HANDLINGAND USUALLY RESULT IN CELL DEATH. THESE LIMITATIONS MUST BEOVERCOME IF CELL CULTURE APPLICATIONS ARE TO BE REALIZED IN MICROGRAVITY ENVIRONMENTS OF MANNED SPACE STATIONS. OPTRA PROPOSES TO DEVELOP AN AUTOMATED INSTRUMENT FOR MONITORING THE GROWTH OF CELL CULTURES IN-SITU AND WITH CAPABILITIES OFQUANTIFYING PARTICULATE CELLULAR PROBES SUCH AS ANTIBODIES LABELLED WITH HEAVY METAL COLLOIDS. THE MEASUREMENT METHODS WILL BE BASED ON PRINCIPLES OF LASER LIGHT SCATTERING. THE OBJECTIVES TO DEMONSTRATE FEASIBILITY OF THE APPROACH WILL BE; TO IDENTIFY SCATTERING COMPONENTS FROMDEAD CELLS SO THAT CULTURE VIABILITY CAN BE ASSESSED; TO DEVELOP MORPHOLOGICAL INDICES BASED ON SCATTERING RATIOS SO THAT CHANGES IN MORPHOLOGY CAN BE DISTINGUISHED FROM CHANGES IN CELL NUMBER; AND TO DEVELOP THE MEANS OF QUANTIFYING PARTICULATE PROBES SO THAT NUMEROUS CELLULAR MOLECULES CAN BE MEASURED BY IMMUNOASSAY. THE THEORETICAL BASIS FOR THE PROPOSED WORK HAS BEEN ESTABLISHEDAND A SUCCESSFUL COMPLETION OF THE OBJECTIVES IS ANTICIPATEDUSING A SOUND EMPIRICAL APPROACH. THE PROPOSED INSTRUMENT WILL FACILITATE DEVELOPMENT OF CELL CULTURE APPLICATIONS IN SPACE AND WILL BE A SIGNIFICANT INNOVATION FOR LAND BASED LABORATORIES.

Principal Investigator:

Bruce crary ph d

Business Contact:

Small Business Information at Submission:

Optra Inc
83 Pine St Peabody, MA 01960

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No