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OrthoSwith Probes for High Throughput Screening

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66458
Program Year/Program:
2004 / STTR
Agency Tracking Number:
GM068413
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ORTHOSYSTEMS, INC.
100 Intrepid Lane Suite 1 Syracuse, NY 13205-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2004
Title: OrthoSwith Probes for High Throughput Screening
Agency: HHS
Contract: 2R42GM068413-02
Award Amount: $751,587.00
 

Abstract:

DESCRIPTION (provided by applicant): This project aims at creating and using the tools to screen large libraries of chemical compounds to find and evaluate drug candidates for three targets in HIV-1. These are (1) the RNAnucleocapsid (NC) protein complex, (2) the TAR RNA-Tat protein-CyclinT1 protein complex, and (3) the Vif protein. No inhibitors of these targets are available in current AIDS therapies, in phase I of this project we succeeded in creating a molecular switch that changes its state in the presence of NC, and switches back in the presence of an inhibitor that mimics the viral RNA packaging signal. The inhibitor causes an increase in fluorescence within seconds that is easily monitored in high throughput screens (HTS) of candidate drugs. It is easy and unambiguous to verify that the complex occurs with a 1:1 ratio of protein and switch components (or not), and to determine the affinity constant, K/d. We propose limited prototyping and to create HTS- and Kdkits that others can use to discover and refine drug candidates to interrupt NC, Tat, and Vif function in HIV-I. In the future, similar assays will target all fifteen HIV proteins with new drugs.

Principal Investigator:

Philip N. Borer
3154435925
PNBORER@SYR.EDU

Business Contact:

Laura Brvenik-borer
3156332423
LAURAJBB@ORTHOSYSTEMS.COM
Small Business Information at Submission:

ORTHOSYSTEMS, INC.
ORTHOSYSTEMS, INC. 2-212 CENTER FOR SCIENCE & TECHNOLOGY SYRACUSE, NY 13244

EIN/Tax ID: 020022049
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
SYRACUSE UNIVERSITY
Office of Sponsored Programs
SYRACUSE, NY 13244
RI Type: Nonprofit college or university