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DEVELOPMENT OF ALTERNATIVE, EXOGENOUS METABOLISM SYSTEMS FOR

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
3159
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
3159
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
OXFORD BIOMEDICAL RESEARCH, INC.
2165 AVON INDUSTRIAL DRIVE ROCHESTER HILLS, MI 48309-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1986
Title: DEVELOPMENT OF ALTERNATIVE, EXOGENOUS METABOLISM SYSTEMS FOR
Agency: HHS
Contract: N/A
Award Amount: $374,000.00
 

Abstract:

THE IDENTIFICATION OF MUTAGENIC COMPOUNDS IS AN IMPORTANT SOCIETAL GOAL. SHORT-TERM TESTS THAT EMPLOY MAMMALIAN ENZYMES (GENERALLY MIXED-FUNCTION OXIDASES FROM RAT LIVER) TO PROVIDE METABOLIC ACTIVATION OF PREMUTAGENS TO ULTIMATE MUTAGENS HAVE BEEN DEVELOPED FOR THIS PURPOSE. IT IS NOW CLEAR THAT SOME COMPOUNDS ARE ACTIVATED BY OTHER OXIDIZING ENZYMES, SUCH AS PEROXIDASES, THAT ARE NOT PRESENT IN RAT LIVER S-9 PREPARATIONS. THE USE OF PEROXIDASES IN ROUTINE MUTAGENICITY SCREENING HAS BEEN LIMITED BY THE COMMERCIAL UNAVAILABILITY OF MAMMALIAN PEROXIDASES. WE PROPOSE TO DEVELOP AND COMMERCIALIZE A SHORT-TERM MUTAGENICITY ASSAY THAT EMPLOYS A MAMMALIAN PEROXIDASE TO PROVIDE METABOLIC ACTIVATION AND L5178Y MOUSE LYMPHOMA CELLS TO DETECT MUTAGENS. IN PHASE I, WE WILL DETERMINE THE CONDITIONS UNDER WHICH A SERIES OF AROMATIC AMINES ARE OXIDIZED TO THE SAME EXTENT BY HIGHLY PURIFIED PROSTAGLANDIN H SYNTHASE. THESE CONDITIONS WILL BE USED TO SCREEN THE SAME SERIES OF AROMATIC AMINES FOR MUTAGENICITY IN PHASE II. IN ADDITION TO THE DETERMINATION OF CONDITIONS TO BE USED IN MUTAGENESIS ASSAYS, THE PHASE I RESEARCH WILL PROVIDE VALUABLE DATA ON THE EXTENT OF METABOLISM

Principal Investigator:

Arthur Bull
Principal Investigator
3136285104

Business Contact:

Small Business Information at Submission:

Oxford Biomedical Research Inc
1600 Hosner Rd. Oxford, MI 48051

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No