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PEROXIDASE ACTIVATION

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4947
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4947
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
OXFORD BIOMEDICAL RESEARCH, INC.
2165 AVON INDUSTRIAL DRIVE ROCHESTER HILLS, MI 48309-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: PEROXIDASE ACTIVATION
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

ASSAYS THAT EMPLOY MAMMALIAN ENZYMES (GENERALLY MIXED- FUNCTION OXIDASES FROM RAT LIVER) TO PROVIDE METABOLIC ACTIVATION OF PREMUTAGENS TO ULTIMATE MUTAGENS HAVE BEEN DEVELOPED AND ARE WIDELY USED TO IDENTIFY MUTAGENIC COMPOUNDS. IT IS NOW CLEAR THAT SOME COMPOUNDS ARE ACTIVATED BY OTHER OXIDIZING ENZYMES, SUCH AS PEROXIDASES, THAT ARE NOT PRESENT IN RAT LIVER S-9 PREPARATIONS. HOWEVER, THE USE OF PEROXIDASES IN ROUTINE MUTAGENICITY SCREENING HAS BEEN LIMITED BY THE COMMERCIAL UNAVAILABILITY OF MAMMALIAN PEROXIDASES. THIS PROJECT WILL DEVELOP AND COMMERCIALIZE A SHORT-TERM MUTAGENICITY ASSAY THAT EMPLOYS AMAMMALIAN PEROXIDASE TO PROVIDE METABOLIC ACTIVATION AND SALMONELLA TYPHIMURIUM TO DETECT MUTAGENS. IN PHASE I, RESEARCHERS WILL DEVELOP CONDITIONS TO MAXIMALLY ACTIVATE A SERIES OF AROMATIC AMINES TO MUTAGENS BY HIGHLY PURIFIED PROSTAGLANDIN H SYNTHASE. THE SAME CONDITIONS WILL BE USED IN PHASE II TO SCREEN A VARIETY OF STRUCTURALLY UNRELATED COMPOUNDS FOR PEROXIDASE-INDUCED MUTAGENICITY. THESE EXPERIMENTS WILL PROVIDE THE BASIS FOR A NEW MUTAGENICITY SCREENING SYSTEM BASED ON AN ALTERNATIVE PATHWAY OF METABOLIC ACTIVATION.

Principal Investigator:

Arthur w bull, jr
PRINCIPAL INVESTIGATOR
3136285104

Business Contact:

Small Business Information at Submission:

Oxford Biomedical Research Inc
Po Box 522 1600 Hosner Road Oxford, MI 48051

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No