USA flag logo/image

An Official Website of the United States Government

ENDOGENOUS ACTIVATION OF PREMUTAGENS BY L5178Y CELLS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11471
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11471
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Oxford Medilog Inc
11526 53rd St N Clearwater, FL 33520
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: ENDOGENOUS ACTIVATION OF PREMUTAGENS BY L5178Y CELLS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO GENERATE A MAMMALIAN CELL LINE THAT ENDOGENOUSLY EXPRESSES METABOLICALLY ACTIVE PROSTAGLANDIN H (PGH) SYNTHASE, A PEROXIDASE ENZYME KNOWN TO BE INVOLVED IN MUTAGEN ACTIVATION. AVAILABILITY OF SUCH A CELL LINE WOULD OVERCOMELIMITATIONS OF CURRENT MUTAGENESIS TESTING METHODS AND LEAD TO RESULTS MORE APPLICABLE TO HUMAN RISK ASSESSMENT. TRANSFECTION OF THE MOUSE LYMPHOMA CELL LINE L5178Y/TK(+/-),A CELL LINE SUITABLE FOR USE IN MUTAGENESIS TESTS, WITH AN AVAILABLE CDNA CLONE CONTAINING THE PGH SYNTHASE GENE WILL BE USED TO GENERATE A STABLY TRANSFECTED CELL LINE POSSESSING THE CHARACTERISTICS DESIRED. IT IS EXPECTED THAT PHASE I OF THIS PROJECT WILL RESULT IN THE PRODUCTION OF A CELL LINE THAT EXPRESSES STABLE AND FUNCTIONALLY ACTIVE PGH SYNTHASE. LOGICAL DEVELOPMENTS FOR PHASE II WORK INCLUDE: (1) SUBCELLULAR LOCALIZATION OF THE EXPRESSED PGH SYNTHASE IN THE TRANSFECTED LYMPHOMA CELLS, AND (2) SCREENING OF CHEMICALS THAT MAY REQUIRE PEROXIDASE ACTIVATION TO EXHIBIT THEIR MUTAGENICITY.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Oxford Medilog Inc
11526 53rd St N Clearwater, FL 33520

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No