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Glutamate Biosensors for Ischemia or Single-Cell Studies

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
71801 		Program Year/Program:
2004 / SBIR
Agency Tracking Number:
NS047955 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
PINNACLE TECHNOLOGY, INC
2721 OREGON ST LAWRENCE, KS 66046-4947
View profile »
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Glutamate Biosensors for Ischemia or Single-Cell Studies
Agency: HHS
Contract: 1R43NS047955-01A1
Award Amount: $98,060.00
 

Abstract:

DESCRIPTION (provided by applicant): Pinnacle Technology and Dr. George Wilson of the University of Kansas will design a sensor suite capable of measuring physiologically relevant levels of glutamate, in vivo, in untethered small animals, with high spatial resolution and under ischemic conditions. Research has shown that the major cause of cell death following an ischemic stroke is not hypoxia, but radically elevated levels of glutamate (glutamate excitotoxicity). The ischemic event has been well characterized in terms of blood flow and tissue oxygenation, but other than microdialysis, there is no tool currently available to directly measure glutamate excitotoxicity. Microdialysis is of limited use due to its poor temporal resolution. Electrochemical sensors work well for electroactive species, but not for non-electroactive species. Behavioral scientists also seek to make connections between the brain and behavior. For these studies high spatial resolution is needed. In Phase I, needle-type biosensors (180 mu/m diameter) will be developed to detect large concentrations of glutamate during ischemic conditions. High spatial resolution biosensors (76 mu/m diameter), will also be developed for normal oxygen conditions ( >40 torr). In Phase II, the team will develop a 76 mu/m ischemic glutamate sensor and 10 um glutamate sensors for single-cell measurements and associated electronics.

Principal Investigator:

David A. Johnson
7858328866
DAVEJ@PINNACLET.COM

Business Contact:

Donna Johnson
7858328866
DONNAJ@PINNACLET.COM
Small Business Information at Submission:

PINNACLE TECHNOLOGY, INC.
PINNACLE TECHNOLOGY, INC. 619 E 8TH ST, STE D LAWRENCE, KS 66044

EIN/Tax ID: 742818617
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No