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Liquid crystal based enzyme activity assay

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
70670
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
CA108078
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
PLATYPUS TECHNOLOGIES, LLC
5520 Nobel Drive Suite 100 Madison, WI 53711-4910
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Liquid crystal based enzyme activity assay
Agency: HHS
Contract: 1R43CA108078-01
Award Amount: $400,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The long-term goal of this Phase 1 SBIR is to develop optimized components (nanostructured substrates) and methods for use in the creation of a novel class of rapid, liquid crystal based assays capable of reporting enzyme activity. This proposal focuses on the activity of matrix metalloproteinases because of their central importance to cancer biology. There is a need for new technologies that are more cost effective, less labor intensive, consume less reagents, can be performed in less time, can distinguish between the total amount of MMP present and its activated fraction, and are amenable to high throughput formats. Platypus TM technology can be the foundation for such assays to accelerate basic research, drug discovery, evaluation of anti-cancer drugs and development of prognostic indicators. The combination of nanostructured substrates with liquid crystals requires no secondary labels or additional reagents to report enzyme activity. It will provide a unified platform for many diverse assays relevant to cancer biology. In this proposal, we will fabricate substrates with nanoscale topography by two methods: oblique deposition of thin films of gold and by rubbing of covalently immobilized enzyme substrates that introduce nanoscale order into the surface of the protein film. We will comparatively evaluate the ability of these substrates to: align liquid crystals; determine their tolerance to the presence of non-specific adsorption of proteins in cell media and to allow rapid quantification of matrix metalloproteinase activity. Additionally, we will explore strategies that allow specific identification of MMP-9 and its activated fraction. At the completion of these studies, we will have optimized the core component of a new technology for rapid, cost effective and accurate quantification of enzyme activity.

Principal Investigator:

Barbara A. Israel

Business Contact:

Susan Pschorr
6082652454
SPSCHORR@PLATYPUSTECH.COM
Small Business Information at Submission:

PLATYPUS TECHNOLOGIES, LLC
PLATYPUS TECHNOLOGIES, LLC 505 S ROSA RD, STE 150 MADISON, WI 53719

EIN/Tax ID: 391992935
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No