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Proteases and tags for protein purification and analysis.

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
79876
Program Year/Program:
2006 / SBIR
Agency Tracking Number:
GM076786
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
POTOMAC AFFINITY PROTEINS, LLC
11305 DUNLEITH PL NORTH POTOMAC, MD -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2006
Title: Proteases and tags for protein purification and analysis.
Agency: HHS
Contract: 1R43GM076786-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The overall goal to this project is to create an integrated system for the expression, detection and rapid purification of proteins from cloned genes. The foundation for this system is a highly engineered subtilisin which hydrolyzes substrates in response to a chemical trigger. The subtilisin prodomain (protag) will be developed both for detection of native target protein and as the ligand for affinity purification. The engineered processing subtilisin (psub) will be used both as the binding molecule for affinity purification of protagged fusion proteins and as the processing protease for protag removal. The long range objective is to develop a sophisticated set of tools to facilitate proteomic analysis in the way restriction endonucleases have been used to facilitate genomic analysis.

Principal Investigator:

Biao Ruan
3016109687
ruan@umbi.umd.edu

Business Contact:


3016109687
Small Business Information at Submission:

POTOMAC AFFINITY PROTEINS, LLC
POTOMAC AFFINITY PROTEINS, LLC 11305 DUNLEITH PL NORTH POTOMAC, MD 20878

EIN/Tax ID: 202913258
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No