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Engineered proteases for proteomics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
94107
Program Year/Program:
2009 / SBIR
Agency Tracking Number:
RR025973
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
POTOMAC AFFINITY PROTEINS, LLC
11305 DUNLEITH PL NORTH POTOMAC, MD -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2009
Title: Engineered proteases for proteomics
Agency: HHS
Contract: 1R43RR025973-01
Award Amount: $199,999.00
 

Abstract:

DESCRIPTION (provided by applicant): Proteomics is a rapidly expanding field but current methodologies remain inadequate for achieving its full potential. The most basic enzymological tool for characterizing proteins is the protease. Proteases are already an essential part of proteomic analysis but more sophisticated tools are needed to identify low abundance proteins in highly complex samples. We have engineered a prototype restriction protease which is active in denaturing conditions and which cuts spe cifically at a well-defined cognate sequence motif. Our basic innovation would be the ability to parse a proteome into sequence-edited slices and to detect these edited portions with high resolution and high sensitivity. In Phase I we propose to test the p rototype restriction protease for its suitability in proteomic analysis and to implement a novel directed evolution methodology for the selection of proteases that cut new sequence motifs. The long range objective is to develop a sophisticated set of prote ase tools to facilitate proteomic analysis in the way restriction endonucleases have facilitated genomic analysis. PUBLIC HEALTH RELEVANCE: The complex and dynamic nature of proteomes make them rich with useful information but difficult to characterize. Ou r long-range objective is to develop a sophisticated set of protease tools to facilitate proteomic analysis in the way restriction endonucleases have facilitated genomic analysis. Better proteomic tools will lead to earlier detection of disease states, bet ter treatments, better predictability of the effects of various treatments, and the development of individualized therapies.

Principal Investigator:

Biao Ruan
3016109687
RUAN@UMBI.UMD.EDU

Business Contact:

Bonnie Bryan
potomac_affinity@msn.com
Small Business Information at Submission:

POTOMAC AFFINITY PROTEINS, LLC
POTOMAC AFFINITY PROTEINS, LLC 11305 DUNLEITH PL NORTH POTOMAC, MD 20878

EIN/Tax ID: 202913258
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No