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NONCONTACT MEMBRANE PROTEIN PROBE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
61075
Program Year/Program:
2002 / STTR
Agency Tracking Number:
MH065808
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Prairie Technologies, Inc.
P.O. Box 620677 3030 Laura Lane Suite # 140 Middleton, WI -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: NONCONTACT MEMBRANE PROTEIN PROBE
Agency: HHS
Contract: 1R41MH065808-01
Award Amount: $427,888.00
 

Abstract:

Reading the conformational state of membrane proteins is central to understanding their role in cell signaling, to using them as sensor elements and for screening candidate drug compounds that are targeted toward this important class of biomolecules. While the patch clamp technique for doing this is ubiquitous, it does have significant limitations: two electrodes are required to measure current flow, the GW input impedance combines with unavoidable stray capacitance to limit the amplifier's bandwidth (typically to -10 kHz), and the GW seal required at the cell membrane precludes scanning to image both distribution and dynamics of membrane proteins. To address these limitations, we will develop near-field probes to confine high-frequency excitation to sub-wavelength proportions, enabling interaction with single membrane proteins. Since there are inherent dielectric contrast mechanisms available in protein-lipid systems, these can be used at high frequencies to provide a new method of protein readout. The overall goal of this work is to build scanning protein probes that can image distribution and dynamics of ion channel activity simultaneously.

Principal Investigator:

Daniel Vanderweide
6082656561
DANVDW@ENGR.WISC.EDU

Business Contact:

Michael Szulczewski
6086620022
PRAIRIET@TDS.NET
Small Business Information at Submission:

PRAIRIE TECHNOLOGIES, INC.
PRAIRIE TECHNOLOGIES, INC. 3230 DEMING WAY, STE 175 MIDDLETON, WI 53562

EIN/Tax ID: 391848666
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
UNIVERSITY OF WISCONSIN
UWI
MADISON, WI 53706
RI Type: Nonprofit college or university