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Isopeptide inhibitors for cancer chemotherapy

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
88881
Program Year/Program:
2008 / SBIR
Agency Tracking Number:
CA132246
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
PROGENRA, INC.
271A GREAT VALLEY PKWY 271A GREAT VALLEY PKWY MALVERN, PA 19355-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2008
Title: Isopeptide inhibitors for cancer chemotherapy
Agency: HHS
Contract: 1R43CA132246-01
Award Amount: $192,441.00
 

Abstract:

DESCRIPTION (provided by applicant): Various ubiquitin and ubiquitin-like protein pathway enzymes have been shown by biochemical and genetic evidence to be involved in disease states and are thus regarded as molecular targets for drug discovery. Regarding neoplastic disease, for example, certain de-ubiquitinases (DUBs) are present in large amounts in malignant but not non-malignant cells or tissues and their functions have been linked to defined aspects of the transformed state. Progenra has developed an is opeptidase assay platform that can detect cleavage activity of one class of enzyme -- proteases of ubiquitin or ubiquitin-like protein fusions. The assay is based on the fusion of the amino terminus of a reporter enzyme requiring a free N-terminus for acti vity to the C-terminus of ubiquitin or a ubiquitin-like protein (UBL) such as NEDD8. Isopeptidase cleavage at the fusion junction liberates the active reporter. The assay has been validated for several DUBs and will be used to conduct a screening campaign to find inhibitors of DEN 1, an isopeptidase that cleaves the ubiquitin-like protein NEDD8 from its partner protein BCA3 (breast cancer associated protein 3), blocking the ability of BCA3 to prevent NFkB-induced activation of its pro-survival, anti-apoptot ic transcription program. Inhibitors of DEN 1 are expected to permit BCA3 to block the pro-survival, anti-apoptotic program activated by NFkB in tumor cells treated with radiation or chemotherapeutic agents and thus to be candidates for single agent or adj uvant anticancer therapeutics. The introduction of such therapeutics to market is the commercial goal of this project. To find such inhibitors, the DEN1 assay will first be validated for high throughput screening. Next, two chemical libraries - a targeted protease inhibitor collection and a general diversity set - will be screened. Finally, selected positives from the screen will be evaluated for preclinical development (potency, selectivity, functional activity). Enzymes of the ubiquitin (and ubiquitin-lik e protein) pathway have become popular targets for drug discovery. The association of NEDD8, a ubiquitin-like protein, with another protein called BCA3 helps to reverse the malignant state by causing the cancer cells to die (apoptosis); this effect is magn ified in the presence of many anticancer agents. A third protein called DEN 1 removes NEDD8 from association with BCA3 and thereby promotes the malignant state. An inhibitor of DEN 1 should prevent this interruption of NEDD8-BCA3 association and thereby sh ould be useful in reversing cancers, particularly if given in concert with an established anticancer agent. Progenra proposes to find inhibitors of DEN 1 by screening selected chemical libraries using the assay it has developed. Compounds that are shown by the assay to inhibit DEN 1 will be evaluated further, and the best of these will be proposed for further development as antitumor agents.

Principal Investigator:

Business Contact:


mattern@progenra.com
Small Business Information at Submission:

PROGENRA, INC.
271A Great Valley Parkway MALVERN, PA 19355

EIN/Tax ID: 061660578
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No