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Novel Polymorphic Markers for Genetic Analyses

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
25199
Program Year/Program:
1996 / SBIR
Agency Tracking Number:
25199
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
PROMEGA CORP.
2800 Woods Hollow Road Madison, WI 53711
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1996
Title: Novel Polymorphic Markers for Genetic Analyses
Agency: HHS
Contract: 1 R43 MH52940-1,
Award Amount: $700,918.00
 

Abstract:

Our research will identify and develop a new type of polymorphic DNA marker that will allowsimplification and acceleration of genetic analyses. These multiplex-compatible amplifiable markers willhave the advantage of simplified analysis versus current PCR-based marker systems, allowing detectionin a new agarose gel format rather than requiring denaturing polyacrylamide gels. The markers will alsobe amenable to fluorescent analysis rather than requiring radioactive detection. In phase l, the plan isto describe a method to identify large-numbers of the new markers and to isolate and characterizeseveral examples. In addition, the basic expectations that they will produce precise amplificationproducts and that their allelic content can be determined more efficiently, safely, and cheaply than othersystems will be demonstrated. This will establish the superiority of this marker class and support thework of phase Il which includes isolation of a large number of the markers, more thorough characteriza-tion of their polymorphic content, and application in generation of linkage maps. In phase I, fluorescentdetection methods will be surveyed and selected to demonstrate that the improved "allele quality"simplifies detection enhancing the process of automated data retrieval and analysis using a newfluorescent imaging reader. The result will establish the feasibility of the phase ill goal of developmentof a fully integrated system of amplification, simplified electrophoretic separations, fluorescent detection,and automated data analysis.

Principal Investigator:

James Schumm
6082744330

Business Contact:

Small Business Information at Submission:

Promega Corp.
2800 Woods Hollow Road Madison, WI 53711

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No