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Developing Technologies for Studying Protein Prenylation

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29458
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
29458
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
PROMEGA CORP.
2800 Woods Hollow Road Madison, WI 53711
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1995
Title: Developing Technologies for Studying Protein Prenylation
Agency: HHS
Contract: 1 R43 GM52807-01,
Award Amount: $98,782.00
 

Abstract:

Prenylation (the modification of proteins with the cholesterol biosynthesis intermediates C(15) fargeranylgeranyl) is a newly discovered postranslational modification that targets certain proteins tointerior of the cell, thereby regulating their biological activity. The best studied example illustrmodification is the farnesylation of p21ras, the protein encoded for by the ras oncogene which is imnumber of human cancers. Farnesylation of p21ras is required for the plasma membrane localization anactivity of this protein. Our primary goal is to generate antisera that specifically recognize the fmoiety. Antibodies to prenylated cysteine (which are not currently available) will allow rapid detecproteins using standard procedures (e.g., SDS-PAGE, Western blotting, immunoprecipitation, ELISA). Tinclude (i) synthesize prenylated amino acid and peptide derivatives, (ii) couple prenylated derivatand (iii) immunize chickens and rabbits and screen sera for antibodies that recognize farnesylated aproteins.

Principal Investigator:

Erik Schaefer
60874

Business Contact:

Small Business Information at Submission:

Promega Corp.
2800 Woods Hollow Road Madison, WI 53711

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No