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QUANTITATION OF STEROID RECEPTORS USING IMAGE ANALYSIS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: CA72556-01A1
Agency Tracking Number: 39224
Amount: $86,033.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1997
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
925 S ROUTE 83
Elmhurst, IL 60126
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 BACUS, SARAH S
 () -
Business Contact
Phone: (708) 993-0204
Research Institution
N/A
Abstract

The objective of this project is to develop quantitative estrogen (ER) and progesterone receptor (PgR) measurements using high resolution digital image microscopy combined with immunohistochemistry techniques. These techniques will enable direct measurements of receptor amounts in tissues and cells. The use of image analysis for quantitation of estrogen and progesterone receptors in the clinical pathology laboratory is rapidly becoming an established test. Commercially available image analysis assays only give the percentage of cells that express the receptors, without taking into account the amount of receptors in the cells; thus, are incapable of giving quantitative amounts of steroid receptors. The assay proposed will 1) produce cell pellets with known amounts of steroid receptors (or progesterone receptors) 2) calibrate the imaging system to report estrogen receptor levels (or progesterone receptor levels) using the cell pellets on which the amount of receptors have been pre- determined, and 3) measure the concentration of receptors in tissues, and report the amount of receptors on breast cancer tissue in fmol/mg equivalents of protein.

* Information listed above is at the time of submission. *

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