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DETECTING DNA DAMAGE IN INDIVIDUAL GERM CELLS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13766
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13766
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Rao Enterprises Inc
Po Box 13501 Res Triangle Park, NC 27709
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: DETECTING DNA DAMAGE IN INDIVIDUAL GERM CELLS
Agency: HHS
Contract: N/A
Award Amount: $49,000.00
 

Abstract:

A RECENTLY DEVELOPED MICROGEL ALKALINE ELECTROPHORESIS TECHNIQUE PROVIDES AN EXTREMELY SENSITIVE METHOD FOR EVALUATING THE PRESENCE OF DNA DAMAGE (SINGLE-STRAND BREAKS AND/OR ALKALI-LABILE SITES) IN INDIVIDUAL CELLS. BECAUSE EXCISION REPAIR PROCESSES ARE NOT REQUIRED TO DEMONSTRATE THE PRESENCE OF DNA LESIONS AND BECAUSE SAMPLE SIZES CAN BE EXTREMELY SMALL (1 TO <10(5)), THE SINGLE-CELL GEL TECHNIQUESHOULD BE SUPERIOR TO OTHER METHODS CURRENTLY USED TO EVALUATE DNA DAMAGE IN GERM CELLS. FURTHERMORE, BECAUSE DATA ARE COLLECTED AT THE LEVEL OF THE SINGLE CELL, AN ASSESSMENT OF INTERCELLULAR DIFFERENCES IN DAMAGE CAN BE CONDUCTED, MAKING IT MORE INFORMATIVE THAN BIOCHEMICAL TECHNIQUES BASED ON POOLED CELL SAMPLES. THE PURPOSE OF THIS PROJECT IS TO EVALUATE THE SUITABILITY OF THIS TECHNIQUE FOR DETECTING DNA DAMAGE IN THE CELLS OF MALE MICE EXPOSED TO POSSIBLE MUTAGENS. THE FOCUS OF THE PROJECT WILL BE ON THE VARIOUS METHODS USED TO ISOLATE GERM CELLS FROM THE TESTIS AND THE KINETICS OF THE INDUCTION OF DNA DAMAGE INDUCED BY SELECTED MUTAGENIC AGENTS (ACRYLAMIDE,CIS-PLATINUM, CYCLOPHOSPHAMIDE, AND METHYLMETHANESULFONATE).

Principal Investigator:

Raymond Tice
9195445857

Business Contact:

Small Business Information at Submission:

Rao Enterprises Inc
Po Box 13501 Res Triangle Park, NC 27709

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No