A method to discover high concentrations of white blood cells in the human body is essential to determine the location of infections of unknown origin (occult abscesses) and for other conditions. present methods are expensive, time-consuming, and require significant technical expertise.this project… More

A quality control (qc) test kit will be developed and and validated for use in measuring the percentage of immunoreactive radioactivity of radiolabeled antibody or antibody fragments intended for patient use. this qc product is being designed for a multicentered clinical trialof compounded,… More

A dot immunoassay for the detection of acrosome reaction of human sperm will be developed for use in the detection of male infertility. screening tests for the determination of the causes of infertility and screening semen samples for sperm banking have become major responsibilities of fertility… More

A new method of direct labeling of antibodies with technetium-99m (tc-99m) will be developed and evaluated. inthis method, called the regulated reduction method, one chemical means is used to break the disulfide bond in the protein, and a separate chemical means is used to reduce the sodium… More

The objective of the study is to develop a pan-t-lymphocyte 99mtc-radiotracer which can be used to study trafficking of stimulated lymphocytes and as a diagnostic radiopharmaceutical for chronic infections such as osteomyelitis and for granulomatous diseases. the plant-derived isolectin,… More

Antibodies or antibody fragments, reduced and lyophilized for use in preparing instant, one-step tc-99m radiopharmaceuticals, can also be labeled with cu-67. the cu-67, as cu(ii) in a ph 5.6 buffer, is simply added to the antibody to generate the cu-67-labeled antibody. the purpose of this project… More

Opportunistic infections are a major cause of morbidity and mortality in aids patients. although it is generally believed that the early diagnosis and treatment of an opportunistic infection is critical to decreasing morbidity and increasing overall survival, localizing the site of these infections… More

Formulated chemotactic peptides, with endogenous tc-99m binding sites, will be developed for use in localizing abscesses, inflammations, and infections. the peptides are designed to have the biological function located at one end of the molecule, and the tc-99m binding site at the other end of the… More

The objective is development of a radiopharmaceutical, using a synthetic laminin nonapeptide radiolabeled with tc-9m, for detection and localization of metastatic tumor cells of epithelial origin. the laminin nonapeptide will be used to target the laminin receptor; laminin receptors are known to… More