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Protein Production in Tobacco from an Amplified Inducible System

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
78071
Program Year/Program:
2006 / STTR
Agency Tracking Number:
A064-015-0073
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
RXOA BIOSCIENCES LLC
1212 FOURIER DR STE 200 Madison, WI 53717-1961
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2006
Title: Protein Production in Tobacco from an Amplified Inducible System
Agency / Branch: DOD / ARMY
Contract: W911NF-06-C-0105
Award Amount: $99,438.00
 

Abstract:

This Phase I proposal will test a UV-B light inducible system in tobacco to produce exceptionally large amounts of protein. There are three principal components to the system: 1) a tightly regulated, UV-B inducible promoter fused to a geminivirus replication associated protein (Rep) coding region, 2) a geminivirus-derived vector that is rapidly amplified by the Rep protein to produce very high yields of the target protein, and 3) plant growth under light-emitting diode (LED) lighting for precise control of the expression system. After sufficient plant biomass accumulation, exposure to UV-B will trigger production of Rep. The Gemini vector DNA will then be precisely excised from the plant genome and replicated episomally, leading to very high intracellular concentrations of transgene mRNA and the encoded transgenic protein product. The specific aims of the Phase I proposal are to: 1 Assemble UV-B inducible genetic constructs to express Rep protein and introduce them into tobacco plants. 2 Test a prototype LED-based lighting system for plant biomass production. 3 Test background expression levels and upon UV-B activation, test the ability of the constructs to induce the geminivirus based expression system in tobacco. 4 Determine the total amount of protein produced per gram of plant biomass.

Principal Investigator:

William L. Petersen
6082292833
williamljpt@aol.com

Business Contact:

William L. Petersen
Managing Member
6082292833
williamljpt@aol.com
Small Business Information at Submission:

RXOA BIOSCIENCES LLC
1212 Fourier Drive Madison, WI 53717

EIN/Tax ID: 134294130
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
ARIZONA BOARD OF REGENTS, ASUNIV.
P.O. Box 873503
Tempe, AZ 85287-3503
Contact: Candyace Lindsay
Contact Phone: (408) 727-0749
RI Type: Nonprofit college or university