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Engineered Revision of the E. coli Genome for Production

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
71165
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
AI055077
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
SCARAB GENOMICS, LLC
1202 Ann St MADISON, WI 53713-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Engineered Revision of the E. coli Genome for Production
Agency: HHS
Contract: 1R43AI055077-01A2
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): E. coli K-12 is well established as a preferred host for commercial production of recombinant DNA products including enzymes, vaccines, metabolites and DNA itself. The complete genome sequence of the E. coli bacterium has now been determined and it shows the presence of many genes that are clearly unnecessary for production purposes and which are arguably detrimental. These include genes encoding phage remnants, adhesins, toxins and ORFs in pathogenicity islands which if activated could adulterate the recombinant product with dangerous substances. Transposible elements and other forms of selfish DNA that render the E. clol genome unstable could also cause difficulties with quality control. Some genes whose expression is simply unnecessary are therefore potentially wasteful. To improve the situation we have deleted over 14% of the E. coli K-12 genome in 39 segments. The reduced genome strain grows at a normal rate in minimal medium. By design it lacks all transposible elements, most candidates for toxic products and many seemingly unnecessary genes. A further reduction of the genome to 19.4% is in progress. In this research we propose to study the properties of the reduced genome E. coli relevant to the use as a production host by comparing the yield, purity and stability of a test product, Green Fluorescent Protein with the undeleted control strains DH10B and the parent MG1655. We also plan to measure the yield of DNA, and mutation frequency, and gene expression levels through DNA microarrays.

Principal Investigator:

Frederick R. Blattner
6082519284
FREDBLATTNER@SCARABGENOMICS.COM

Business Contact:

Frederick Blattner
6082587438
FREDBLATTNER@HOTMAIL.COM
Small Business Information at Submission:

SCARAB GENOMICS, LLC
SCARAB GENOMICS, LLC 1547 JEFFERSON ST MADISON, WI 53711

EIN/Tax ID: 364492509
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No