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LUMINESCENCE DETECTION REAGENTS FOR GLYCOLIPIDS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11104
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
11104
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Sealite Sciences, Inc.
3000 N WOODS PKY, STE 200 Norcross, GA 30071
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1991
Title: LUMINESCENCE DETECTION REAGENTS FOR GLYCOLIPIDS
Agency: HHS
Contract: N/A
Award Amount: $468,906.00
 

Abstract:

ALTERATIONS IN SURFACE CARBOHYDRATES IN GLYCOLIPIDS AND GLYCOPROTEINS OF TUMOR CELLS RELATIVE TO NORMAL CELLS IS A COMMON FEATURE OF MALIGNANT DISEASES. IMMUNOSTAINING PROCEDURES DEVELOPED FOR DETECTING GLYCOLIPID ANTIGENS WITH TUMOR-SPECIFIC ANTIBODIES REPRESENT IMPORTANT NEW TECHNIQUESFOR IMMUNODIAGNOSTICS AND IMMUNOTHERAPEUTICS. THE CURRENT METHODS FOR THESE DIAGNOSTIC TESTS RELY ON REAGENTS THAT ARERADIOACTIVE OR ENZYME LINKED. IN THESE STUDIES, A METHOD WILL BE DEVELOPED FOR ASSAYING GLYCOLIPIDS ON SOLID SUPPORTS THAT IS MORE RAPID AND 3 TO 6 ORDERS OF MAGNITUDE MORE SENSITIVE THAN EXISTING TECHNIQUES AND DOES NOT UTILIZE RADIOISOTOPES. THIS NEW TECHNOLOGY EXPLOITS THE REMARKABLE SENSITIVITY OF THE RECOMBINANT BIOLUMINESCENT PROTEIN, AEQUORIN, AND THE HIGH-AFFINITY INTERACTIONS OF STREPTAVIDIN AND BIOTIN. THIS RESEARCH HAS THREE SPECIFIC AIMS. FIRST, ASSAYS WILL BE ESTABLISHED USING LUMINESCENCE DETECTION REAGENTS (LDR'S)FOR ASSAYING FORSSMAN GLYCOLIPID, A HUMAN TUMOR ANTIGEN, IN MICROTITER PLATES. SECOND, THIS ASSAY WILL BE ADAPTED TO DETECT GLYCOLIPIDS SEPARATED BY THIN-LAYER CHROMATOGRAPHY. THIRD, AN ASSAY WILL BE DEVELOPED FOR DETECTING THE SIALYL-LE(A) ANTIGEN IN COMPLEX MIXTURES USING LDR IN COMBINATION WITH THE COLORECTAL CARCINOMA-SPECIFIC MONOCLONAL ANTIBODY, CA 19-9. THE ASSAYS AND REAGENTS DEVELOPED WILL HAVE IMPORTANT APPLICATIONS IN BOTH BASIC RESEARCH AND CLINICAL LABORATORIES INVOLVED IN THE ANALYSIS OF COMPLEX CARBOHYDRATES.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Sealite Sciences Inc.
Box 6033 Athens, GA 30604

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No