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IN VITRO SCREENING TEST FOR DETECTING ANTIESTROGENS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7168
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7168
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Sitek Research Laboratories
12111 Parklawn Drive Rockville, MD 20852
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: IN VITRO SCREENING TEST FOR DETECTING ANTIESTROGENS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A RELIABLE IN VITRO TEST SYSTEM USING HUMAN CELL LINES TO SCREEN COMPOUNDS FOR THEIR CYTOTOXICITY AND ANTIESTROGENIC ACTIVITY. CURRENTLY, A NUMBER OF IN VITRO SCREENING TESTS ARE USED TO IDENTIFY POTENTIAL CHEMOTHERAPEUTIC DRUGS. MOST OF THESE TESTS UTILIZE NONHUMAN (MOSTLY RODENT) CELL LINES THAT MAY NOT DISTINGUISH SPECIES-SPECIFIC DIFFERENCES IN TOXICITY OF CHEMICALS BETWEEN HUMANS AND RODENTS. ALTHOUGH A FEW HUMAN CELL LINES (HT-29, A549, KB) ARE CURRENTLY USED TO SCREEN CHEMICALS FOR THEIR CYTOTOXICITIES, NONE OF THEM ARE RESPONSIVE TO ESTROGENS OR ANTIESTROGENS. BECAUSE IN RECENTYEARS PATIENTS WITH HORMONE-RESPONSIVE SOLID TUMORS HAVE BEEN TREATED WITH BOTH ENDOCRINE DRUGS (ANTIESTROGENS) AND OTHER CHEMOTHERAPEUTIC AGENTS, IT IS ESSENTIAL TO DEVELOP ANIN VITRO SCREENING TEST WITH APPROPRIATE HUMAN ESTROGEN-SENSITIVE AND ESTROGEN-NONSENSITIVE CELL LINES. USE OF HUMAN CELL LINES WILL ALSO BE MORE RELEVANT IN VIEW OF THE DIFFERENCES IN THE SPECIES-SPECIFIC CYTOTOXICITY OF ANUMBER OF COMPOUNDS. SITEK RESEARCH LABORATORIES PROPOSES TO DEVELOP A RELIABLE PROTOCOL FOR A RAPID, IN VITRO TEST USING ESTROGEN-RESPONSIVE AND ESTROGEN-NONRESPONSIVE HUMAN CELL LINES. SUCH A TEST WILL BE SENSITIVE, RELATIVELY INEXPENSIVE, AND ALLOW THE EVALUATION OF A NUMBER OF SAMPLESAT THE SAME TIME. IN ADDITION, THE TEST WILL DISTINGUISH THE TOXICITY CAUSED BY THE ANTIESTROGENS FROM THE NONSPECIFIC CYTOTOXICITY OR TOXICITY CAUSED BY OTHER CHEMOTHERAPEUTIC DRUGS.

Principal Investigator:

Arulasanam k thilagar phd
3019842302

Business Contact:

Small Business Information at Submission:

Sitek Research Laboratories
12111 Parklawn Drive Rockville, MD 20852

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No