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A RAPID IMMUNOASSAY FOR YERSINIA ENTERCOLITICA

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7885
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
7885
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Skyland Scientific Svcs Inc
2311 S 7th Ave Bozeman, MT 59715
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: A RAPID IMMUNOASSAY FOR YERSINIA ENTERCOLITICA
Agency: HHS
Contract: N/A
Award Amount: $30,000.00
 

Abstract:

FIVE MAJOR FOODBORNE OUTBREAKS OF YERSINIOSIS HAVE OCCURRED IN THE UNITED STATES WITHIN THE LAST DECADE AND THE DISEASE IS ENDEMIC IN MANY OTHER COUNTRIES OF THE WORLD. YERSINIA ENTEROCOLITICA IS OF PARTICULAR CONCERN TO THE FOODINDUSTRY BECAUSE IT IS A PSYCHROTROPH, AND, THEREFORE, GROWTH CAN NOT BE CONTROLLED BY REFRIGERATION. LABORATORY METHODOLOGY PRESENTLY AVAILABLE FOR DETECTING Y. ENTEROCOLITICA IS TIME-CONSUMING. TWO UNIQUE ANTIGENIC STRUCTURES ON PATHOGENIC STRAINS OF Y. ENTEROCOLITICA PRESENT THE BASIS FOR DEVELOPING AN IMMUNOASSAY FOR RAPID DETECTION OF Y. ENTEROCOLITICA: (I) UNIQUE OUTER MEMBRANE PROTEINS SYNTHESIZED AT 37 C BY STRAINS THAT CARRY A VIRULENCE PLASMID, AND (II) FLAGELLA. SPECIFIC ANTIBODY WILL BE INCORPORATED INTO TWO EXPERIMENTAL IMMUNOASSAYS: (I)A DISPERSE DYE SYSTEM, AND (II) AN AVIDIN-BIOTIN ENZYME SYSTEM. AN IMMUNOASSAY CAN BE ASSEMBLED AS A MARKETABLE TEST KIT FOR RAPID DETECTION OF Y. ENTEROCOLITICA IN FOODS. A LONG-TERM OBJECTIVE IS THE DEVELOPMENT OF ANTIGEN AND ANTIBODY DETECTION SYSTEMS FOR RAPID IDENTIFICATION OF Y. ENTEROCOLITICA AND MEASURING SEROLOGICAL RESPONSES IN CLINICAL MICROBIOLOGY.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Skyland Scientific Svcs Inc
2311 S 7th Ave Bozeman, MT 59715

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No