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Multi-dimensional fluorescence microscope

Award Information

National Aeronautics and Space Administration
Award ID:
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Southwest Sciences, Inc.
1570 Pacheco Street Suite E-11 Santa Fe, NM 87505-3993
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 1
Fiscal Year: 2002
Title: Multi-dimensional fluorescence microscope
Agency: NASA
Contract: NAS5-01215
Award Amount: $70,000.00


Southwest Sciences, Inc. proposes development of an innovative five-dimensional fluorescence imaging system that combines spectrally resolved, scanning confocal fluorescence microscopy with fluorescence lifetime detection. Our proposed multi-dimensional fluorescence microscope is unique from previous work because it provides simultaneous measurement of lifetime decay profiles at multiple wavelengths. The combination of spectral and lifetime resolution will provide superior contrast between multiple fluorophores and allow the design of more sophisticated fluorescence experiments for structural and functional imaging of cellular and sub-cellular systems. Complete fluorescence decay profiles will be obtained with one nanosecond resolution at each of 16 wavelengths. The proposed microscope and detection instrumentation are a flexible system that is generally applicable to a wide range of fluorescence microscopy applications. The instrument can be designed to accommodate different excitation and emission wavelengths and is compatible with either single or two photon excitation. A modular design that can accept different excitation methods, optics and detectors can be developed. Diode laser excitation and radio frequency (rf) detection electronics will result in a compact, low power, lightweight instrument.

Principal Investigator:

Kristen Peterson
Principal Investigator

Business Contact:

Alan C. Stanton
Small Business Information at Submission:

Southwest Sciences, Inc.
1570 Pacheco St., Suite E-11 Santa Fe, NM 87505

EIN/Tax ID: 850340320
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No