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MEMBRANE-BOUND RAD P21 REGULATION BY GAP AND LIPIDS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19072
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
19072
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Sphinx Pharmaceuticals
Box 52330, 4 University Place Durham, NC 27717
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: MEMBRANE-BOUND RAD P21 REGULATION BY GAP AND LIPIDS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM GOAL OF THIS PROJECT IS TO DISCOVER DRUGS THAT WILL PROTECT NORMAL CELLS FROM CANCER CHEMOTHERAPY. THE ONCOGENE RAS IS PRESENT IN APPROXIMATELY 30% OF HUMAN TUMORS; A NORMAL NON-ONCOGENIC RAS IS FOUND IN NORMAL CELLS. THIS NORMAL RAS (BUT NOT ONCOGENIC RAS) CAN BE SWITCHED OFF BY THE CYTOSOLIC PROTEIN GAP RESULTING IN INHIBITION OF CELL DIVISION. THIS DIFFERENCE CAN BE EXPLOITED BY COMPOUNDS THAT ACT THROUGH GAP-RAS TO INHIBIT THE PROLIFERATION OF NORMAL CELLS, SPARING THEM FROM THE EFFECTS OF ANTI-PROLIFERATIVE THERAPY. MOST PREVIOUS WORK ON GAP-RES INTERACTIONS HAS UTILIZED SOLUBLE RAS. SINCE LIPIDS APPEAR TO REGULATE THE GAP-RAS INTERACTION, IT IS IMPORTANT TO USE THE MORE PHYSIOLOGICAL MEMBRANE-BOUND FORM OF RAS P21. IN PHASE I OF THIS SBIR GRANT, AN ASSAY WILL BE CONSTRUCTED FOR MEMBRANE-BOUND NON-ONCOGENIC RAS PROTEIN AND ITS REGULATION BY GAP (SPECIFIC AIM 1). THE MEMBRANE-BOUND RAS WILL THEN GE USED TO STUDY THE EFFECTS OF REGULATORY LIPIDS (SPECIFIC AIM 2). SUBSEQUENT TO PHASE I, COMPOUNDS ACTING TO INHIBIT NORMAL RAS FUNCTION WILL BE IDENTIFIED, AND TESTED IN RELEVANT CELL AND ANIMAL MODELS.

Principal Investigator:

Stephen B Bocckino
9194890909

Business Contact:

Small Business Information at Submission:

Sphinx Pharmaceuticals
Po Box 52330 Durham, NC 27717

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No