The objective of this project is to develop a dna sequencing instrument utilizing the pyrophosphate-based method of dna sequencing. this is a new method that does not use electrophoresis, radioactivity, or fluorescence. the method works by measuring pyrophosphate generated by the dna polymerization… More

The objective of this project is to construct a novel expression vector system which will facilitate inexpensive mass production and purification of commercially important enzymes. this will be accomplished by producing a novel fusion protein which can be readily purified to homogeneity by a simple… More

One goal of the human genome project is the automated preparation of plasmid DNA from E. coli for subsequent DNA sequencing and restriction mapping. I have developed a radically new procedure for the preparation of plasmid DNA called Sequential Enzymatic Digestion. This method does not employ… More

Synthetic oligodeoxynucleotides play a central role in molecular biology research, for example,in DNA sequencing, DNA amplification, and hybridization. A novel "one-pot" enzymatic method willreplace the cumbersome and expensive phosphoramidite chemical method. The new method promisesincreased speed,… More