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Construction of an Automated Plasmid Prep Instrument

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
22098
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
22098
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Sybtrel Biotechnology
1500 Edwards Avenue, Suite Q Harahan, LA 70123
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1993
Title: Construction of an Automated Plasmid Prep Instrument
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

One goal of the human genome project is the automated preparation of plasmid DNA from E. coli for subsequent DNA sequencing and restriction mapping. I have developed a radically new procedure for the preparation of plasmid DNA called Sequential Enzymatic Digestion. This method does not employ classical purification steps such as centrifugation, precipitation, extraction with organic solvents, chromatography, or electrophoresis. The method works by the sequential addition of hydrolytic enzymes to an E. coli culture followed by a bead based step for plasmid concentration. The method is readily amenable to automation on commercial liquid handling robots. A proposed small bench top robot employing this method will be capable of producing about one thousand plasmid preparations per day. This purified plasmid DNA will be suitable for applications such as DNA sequencing, restriction endonuclease analysis, PCR, hybridization, cloning, and in vitro transcription.

Principal Investigator:

Edward D. Hyman
5047333849

Business Contact:

Small Business Information at Submission:

Sybtrel Biotechnology
1500 Edwards Avenue Harahan, LA 70123

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No