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A HUMAN IL-1 INHIBITOR FOR THE TREATMENT OF SEPTIC SHOCK

Award Information

Agency:
Department of Defense
Branch:
Navy
Award ID:
9168
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
9168
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Synergen Inc
1885 33rd St Boulder, CO 80301
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: A HUMAN IL-1 INHIBITOR FOR THE TREATMENT OF SEPTIC SHOCK
Agency / Branch: DOD / NAVY
Contract: N/A
Award Amount: $49,326.00
 

Abstract:

WE PROPOSE TO PURIFY AN IL-1 INHIBITOR FROM THE SUPERNATANT OF MONOCYTES CULTURED ON PLATES COATED WITH IGG. THIS INHIBITORY ACTIVITY HAS BEEN SHOWN TO BE ABOUT 22 KILODALTONS IN MOLECULAR WEIGHT AND TO INHIBIT IL-1 IN SEVERAL ASSAYS, NAMELY IL-1 MEDIATED THYMOCYTE PROLIFERATION, COLLAGENASE RELEASE BY ARTICULAR CHONDROCYTES, AND PGE(2) RELEASE BY BOTH SYNOVIAL AND DERMAL FIBROBLASTS. WE WILL PRODUCE LARGE QUANTITIES OF THE INHIBITOR-CONTAINING SUPERNATANT AND PURIFY THE PROTEIN BY CONVENTIONAL CHROMATOGRAPHIC TECHNIQUES. INITIALLY WE WILL USE THE THYMOCYTE-PROFILERATION ASSAY TO MONITOR PURIFICATION BUT WILL IMMEDIATELY BEING DEVELOPING A FASTER PGE(2)-RELEASE ASSAY TO SPEED UP PURIFICATION. WE ALSO WILL BEGIN DEVELOPING MONOCLONAL ANTIBODIES TO THIS MOLECULE. OUR PHASE-I GOALS ARE TO DEVELOP A RAPID ASSAY FOR THIS INHIBITOR AND TO ATTAIN A 400-FOLD PURIFICATION.

Principal Investigator:

Dr charles h hannum
3039386200

Business Contact:

Small Business Information at Submission:

Synergen Inc
1885 - 33rd St Boulder, CO 80301

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No