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THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO UNDERSTAND THEMOLECULAR BASIS OF…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4779
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4779
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
T CELL SCIENCES, INC.
119 FOURTH AVENUE Needham, MA 02194
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO UNDERSTAND THEMOLECULAR BASIS OF T CELL RECOGNITION OF TUMOR ANTIGENS OF LUNG CARCINOMA.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO UNDERSTAND THEMOLECULAR BASIS OF T CELL RECOGNITION OF TUMOR ANTIGENS OF LUNG CARCINOMA. THE SPECIFIC AIMS OF THE PROPOSED STUDY ARE(1) TO CHARACTERIZE THE GENES ENCODING T CELL ANTIGEN RECEPTORS (TCAR) FROM THE TUMOR INFILTRATING LYMPHOCYTES; AND (2) TO INVESTIGATE WHETHER THERE IS A LIMITED EXPRESSIONOF T CELL CLONOTYPES DIRECTED AGAINST TUMOR CELLS AT THE SITE OF LUNG TUMORS. A T CELL CLONOTYPE IS DEFINED BY TCAR,WHICH IS COMPOSED OF AN ALPHA AND A BETA POLYPEPTIDE CHAIN. EACH ALPHA AND BETA CHAIN CONSISTS OF VARIABLE (V) AND DIVERSITY (D), JOINING (J) AND CONSTANT (C) DOMAINS. ESTIMATED TOTAL NUMBERS OF V-BETA GENES OF THE TCAR IN THE MURINE SYSTEM ARE APPROXIMATELY TWENTY. PHASE I EFFORTS WILL INCLUDE (1) AN ANALYSIS OF THE TOTAL V-BETA GENE REPERTOIRE AVAILABLE IN HUMANS AND (2) AN ANALYSIS OF THOSE V-BETA GENES EXPRESSED IN THE TUMOR DERIVED T CELLS. FOR THESE STUDIES, A CDNA LIBRARY WILL BE PREPARED FROM TUMOR- DERIVED T CELLS USING A 5' BETA CONSTANT REGION SEQUENCE AS A PRIMER AND APPROPRIATE PHAGES AS VECTOR. COMPARATIVE ANALYSIS OF THESE TUMOR-DERIVED V-BETA DNA SEQUENCES WITH THE COMPLETE REPERTOIRE OF V-BETA SEUENCES OBTAINED FROM A LARGE PERIPHERAL T CELL LIBRARY WILL BE DONE. THESE DNA SEQUENCE ANALYSES MABE EXTENDED TO THE V-ALPHA GENES AND TO THE D AND J REGIONS OF ALPHA AND BETA GENES. THE MOLECULAR ANALYSIS OF THE TUMOR-DERIVED T CELLS MAY PROVIDE A RATIONAL BASIS FOR DEVELOPING T CELL RECEPTOR-ORIENTED DNA PROBES AND MONOCLONAL ANTIBODIES FOR DIAGNOSING AND MONITORING LUNG CANCER PATIENTS.

Principal Investigator:

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PRINCIPAL INVESTIGATOR
6178642160

Business Contact:

Small Business Information at Submission:

T Cell Sciences Inc
840 Memorial Drive Cambridge, MA 02139

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No