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ENHANCED PRODUCTION OF HIV-1 PROTEASE IN E COLI

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11449
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11449
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Tbc Research Laboratories
950 Boston Ave Longmont, CO 80501
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: ENHANCED PRODUCTION OF HIV-1 PROTEASE IN E COLI
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

HUMAN IMMUNODEFICIENCY VIRUS TYPE I (HIV-1) IS THE ETIOLOGICAGENT OF ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS). THIS PROJECT'S LONG-TERM OBJECTIVE IS TO STUDY THE ACTION OF THE HIV-1 PROTEASE TO DEVELOP SPECIFIC PROTEASE INHIBITORS THAT MIGHT BE ABLE TO HALT THE VIRAL REPLICATION. THIS STUDY IS POSSIBLE ONLY WITH THE AVAILABILITY OF LARGE QUANTITIES OF BIOLOGICALLY ACTIVE HIV-1 PROTEASE. PHASE I EFFORTS WILL DEVELOP A RECOMBINANT DNA SYSTEM TO PRODUCE THE PROTEASE. THE APPROACH TO BE USED WILL INVOLVE THE ASSEMBLY OF A SYNTHETIC GENE CODING FOR THE HIV-1 PROTEASE. THE GENE WILL BE CLONED INTO A PLASMID AND EXPRESSED IN ESCHERICHIA COLI. THE PRODUCTION LEVEL OF THE GENE PRODUCT WILL BE OPTIMIZED SO THAT THE PROTEASE CAN BE ECONOMICALLY PRODUCED. HIGH-LEVEL EXPRESSION OF THE PROTEASE WILL ENABLE PHASE II RESEARCH TO FOCUS ON THE E. COLI FERMENTATION SCALE-UP, PURIFICATION, AND RENATURATION OF THE PROTEASE TO YIELD BIOLOGICALLY ACTIVE HIV-1 PROTEASE.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Tbc Research Laboratories
950 Boston Ave Longmont, CO 80501

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No