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CLONING OF THERMOPHILIC BILIRUBIN OXIDASE GENES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11191
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
11191
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
THERMOGEN,INC.
2501 DAVEY RD WOODRIDGE, IL 60517
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1992
Title: CLONING OF THERMOPHILIC BILIRUBIN OXIDASE GENES
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

THERMOGEN, INC., WILL DEVELOP A THERAPEUTIC BIOREACTOR ABLE TO INACTIVATE BILIRUBIN. THE REACTOR WILL BE BASED ON A THERMOSTABLE BILIRUBIN-DEGRADING ENZYME PRODUCED FROM A GENE CLONED FROM A THERMOPHILIC ORGANISM. THE BIOREACTOR WILL BE ABLE TO DETOXIFY BILIRUBIN BY EXTRACORPOREAL OR INTRACORPOREAL TREATMENT OF THE BLOOD OF PATIENTS SUFFERING FROM LIVER DYSFUNCTION. DURING PHASE I, THERMOGEN, INC., WILL SYSTEMATICALLY SCREEN A LARGE VARIETY OF GRAM-NEGATIVE AND GRAM-POSITIVE PROKARYOTIC THERMOPHILIC ORGANISMS FOR THE PRESENCE OF BILIRUBIN-DEGRADING ENZYMES (PREFERABLY OXIDASES). THE SCREENING METHOD IS BASED ON A SIMPLE SPECTROPHOTOMETRIC ASSAY THAT CAN DISTINGUISH BETWEEN THE SUBSTRATE AND ITS DEGRADATION PRODUCTS. THE CLONING OF BILIRUBIN-DEGRADING GENES FROM THE IDENTIFIED ORGANISMS WILL BE ACCOMPLISHED BY SCREENING GENOMIC BANKS IN ESCHERICHIA COLI FOR THE PRESENCE OF BILIRUBIN-MODIFYING ENZYME ACTIVITIES. PHASE II WILL AIM FOR THE COMPLETION OF THE CLONING OF THE IDENTIFIED GENES, THEIR OVEREXPRESSION IN SUITABLE HOSTS, THE PURIFICATION OF THE PROTEIN PRODUCTS, AND THE EVALUATIONOF THEIR PERFORMANCE IN A VARIETY OF PROTOTYPE BIOREACTORS AND AS PIGMENT GALLSTONE-SOLUBILIZING AGENTS. SUBSEQUENT RESEARCH WILL BE DEVOTED TO THE DEVELOPMENT OF U.S. FOOD ANDDRUG ADMINISTRATION-APPROVED BIOREACTORS AND ENZYME PREPARATIONS FOR EXTRACORPOREAL TREATMENT OF JAUNDICE.

Principal Investigator:


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Business Contact:

Small Business Information at Submission:

Thermogen Inc.
1451 E 55th St Ste 621n Chicago, IL 60615

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No