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Universal System for Directed Nucleic Acid Cleavage

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
25122
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
25122
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
THIRD WAVE TECHNOLOGIES, INC.
THIRD WAVE TECHNOLOGIES, INC. 506 S ROSA RD MADISON, WI 53719
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1994
Title: Universal System for Directed Nucleic Acid Cleavage
Agency: HHS
Contract: 1 R43 GM51704-1,
Award Amount: $75,000.00
 

Abstract:

We will develop a novel system for directed nucleic acid cleavage. This system is based uponour discovery that the 5' nucleases of several thermostable DNA polymerases can be directed to cleaveas site-specific endonucleases. This system will be thermostable, specific, efficient and easilyautomatable, and will find broad application in the fields of nucleic acids-based research and diagnostics.In Phase I we will seek to overcome the most substantial limitation of this technology, its inefficiencyat cleaving long 5' arms. We will examine the effects of reaction constituents and conditions on theefficiency of cleavage of a well-characterized substrate, performing a comprehensive examination of theeffects of different salts, divalent cations, denaturants, buffer pH values, helicases and temperatures.We will also use mutagenesis to alter the protein and influence its interaction with the substratestructure. During Phase II we will optimize the required substrate complex formations and nucleaseinteractions to develop this technology into a generic, directed nucleic acid cleavage system for bothsingle and double stranded nucleic acids.

Principal Investigator:

Maryann Brow
6082380810

Business Contact:

Small Business Information at Submission:

Third Wave Technologies
2800 S Fish Hatchery Road Madison, WI 53715

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No