Autosomal Sex Reversal of Rainbow Trout
The proposed SBIR project seeks to develop an alternative approach to the use of
hormone treatment (17 -methyltestosterone) of broodstock for the production of
all-female rainbow trout. In the course of normal spawning of large numbers of
rainbow trout, Troutlodge personnel have identified rare occurrences of males in
populations of XX female stocks. Previous studies using common carp in the
Netherlands and rainbow trout in France have identified similar rare male
individuals and determined that they are the result of homozygosity for a recessive
autosomal sex-determining allele that overrides the normal XX-XY sex
determination mechanism. This project will test for a similar phenomenon in
Troutlodge stocks of rainbow trout, and utilize these alleles to establish male lines
of XX individuals without the use of testosterone analogs.
Rare males will be tested for (1) their ability to produce all female progeny in
crosses with normal females, and (2) their ability to produce significant numbers of
male progeny when their sperm is used for androgenesis (all-paternal inheritance).
If both objectives are successful, it will indicate that autosomal sex reversal is
likely and lead directly to Phase II research.
Small Business Information at Submission:
PO Box 1290 Sumner, WA 98390
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