Induction of Triploidy and Gynogenesis in Cobia (Rachycentron canadum)
Cobia (Rachycentron canadum) exhibit differential growth rates between the sexes, with females growing faster than males. Sex determination mechanisms have not been elucidated for this species, nor are there any literature reports regarding the induction of sterility through triploidy or attempts at generating uniparental inheritance.
The research outlined in this proposal will establish protocols for the induction of gynogenesis and triploidy in cobia. Specifically we will determine the appropriate methods for suppression of second meiosis in newly fertilized eggs and the protocols needed for inactivating parental sperm DNA.
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