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The Molecular Culture

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
71269
Program Year/Program:
2004 / STTR
Agency Tracking Number:
AI062101
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
TRANSGENOMIC, INC.
12325 Emmet Street Omaha, NE 68164-4268
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: The Molecular Culture
Agency: HHS
Contract: 1R41AI062101-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): In the clinical setting, molecular microbiologic detection methods have classically focused on a single microorganism or a small group of microorganisms. Broad range molecular detection of bacteria in clinical specimens is an approach that parallels a conventional culture-based approach, may be more rapid, and offers the possibility of identifying so-called "non-culturable" organisms. A common molecular target used for broad range detection of bacteria using polymerase chain reaction (PCR) is 16S ribosomal DNA (rDNA). Primers are designed based on areas of 16S rDNA sequence conserved amongst bacteria; sequencing of the amplified DNA yields data which can be used to identify the source organism. Our research collaborator, Dr. Robin Patel, at Mayo Clinic, Rochester, MN, has extensive experience with this approach. Unfortunately, this technique is not applicable to the direct diagnosis of polymicrobial bacterial infections unless the mixed 16S rDNA amplification products can be separated and then separately sequenced. Traditional approaches to separation of mixed amplification products include temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, and cloning; these approaches are cumbersome, especially for use in the routine diagnostic setting. The purpose of this proposal is to evaluate a new application for separation of mixed 16S rDNA amplification products prior to sequencing - denaturing high performance liquid chromatography using the WAVE(r) System (Transgenomic Inc., Omaha NE). This system has been shown to be able to separate PCR products differing by a single nucleotide. The sensitivity of this rapid, high-throughput technique to detect bacteria present in varying ratios in mixed species populations will be assessed. Denaturing high performance liquid chromatography-based profiling and sequence-based investigation of 16S rDNA (or alternate broad range target) PCR amplification products could be applicable to a wide variety of patient specimen types (e.g. synovial fluid, cerebrospinal fluid, blood, abscess material) and diseases (e.g. prosthetic joint infection, meningitis, bacteremia), as well as to characterization of mixed-species bacterial biofilms, normal flora (e.g. gastrointestinal tract, genital tract, oral cavity) and agricultural or environmental specimens.

Principal Investigator:

George Hong
4025102622
GHONG@TRANSGENOMIC.COM

Business Contact:


4024525414
Small Business Information at Submission:

TRANSGENOMIC, INC.
TRANSGENOMIC, INC. 12325 EMMET ST OMAHA, NE 68164

EIN/Tax ID: 911789357
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
MAYO CLINIC COLL OF MEDICINE, ROCHESTER
MAYO CLINIC ROCHESTER
Omaha, MN 55905
RI Type: Domestic nonprofit research organization