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Development of a Fluorometric In Vitro Assay for HIV

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29112
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
29112
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
TREVIGEN, INC.
8405 HELGERMAN COURT GAITHERSBURG, MD 20877-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1995
Title: Development of a Fluorometric In Vitro Assay for HIV
Agency: HHS
Contract: 1 R43 AI37463-01,
Award Amount: $98,224.00
 

Abstract:

The function of retroviral integrase (IN) is the integration of viral DNA into host cell DNA, an esretroviral replication. IN protein recognizes viral cognate at sites, removes two nucleotides from tviral DNA (3' processing) and joins the 3'-processed cognate at sites to the newly created 5' ends otransfer). This research will develop a fluorometric assay utilizing fluorescence resonance energy tprimarily the result of dipole-dipole interactions between the donor and the acceptor. Hence, when afluorophores are introduced to a DNA substrate in close proximity, the donor fluorescence will be quThe process will be fully reversed if fluorophores are physically separated by enzyme-mediated DNA cquenched fluorescence will be recovered and the extent of this change upon cleavage of fluorescent Dreflect the extent of the cleavage reaction of DNA. This assay is non radioactive, sensitive, rapid,possible to obtain precise kinetic information essential for mechanistic studies and screening of en

Principal Investigator:

Philip Vanek
30116

Business Contact:

Small Business Information at Submission:

Trevigen, Inc.
8405 Helgerman Court Gaithersburg, MD 20877

EIN/Tax ID: 521797207
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No