USA flag logo/image

An Official Website of the United States Government

RAPID CANCER PREDISPOSITION SCREEN FOR GENE MUTATIONS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
39261
Program Year/Program:
1997 / SBIR
Agency Tracking Number:
39261
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
TREVIGEN, INC.
8405 HELGERMAN COURT GAITHERSBURG, MD 20877-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1997
Title: RAPID CANCER PREDISPOSITION SCREEN FOR GENE MUTATIONS
Agency: HHS
Contract: CA74639-01
Award Amount: $100,000.00
 

Abstract:

TreviMed has developed a novel patent pending technology, Oscillating Cleavage Amplification Reaction (OsCAR), for the detection of point mutations in clinical samples. The entire assay will take about one hour from preparation of the DNA sample and will utilize capillary electrophoretic separation, which presently takes about two minutes. Presently, the assay detects attomole levels of target using its inherent signal amplification technology. The technology is extremely cost effective and automatable, making it attractive for large scale screening. OsCAR does not require PCR, which has been shown to be problematic for mismatch detection due to the generation of point mutations by the synthetic amplification step. In this proposal, we plan to apply Oscar to the detection of human mismatch repair enzymes implicated in hereditary nonpolyposis colon cancer (HNPCC), which is inherited in an autosomal dominant fashion resulting in colorectal cancer, and less frequently in ovary and endometrial tumors. The large gene size (73 kbp) and organization (16 exons) makes DNA sequencing and other approaches challenging, making OsCAR detection of specific high frequency mutations more practical.

Principal Investigator:

Bazar, leonard s
0

Business Contact:


3012162800
Small Business Information at Submission:

Trevigen, Inc.
8405 HELGERMAN CT Gaithersburg, MD 20877

EIN/Tax ID: 521797207
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No