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Diagnosing DNA Mutations Using Mismatch Repair Enzymes

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
65265
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
CA083441
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
TREVIGEN, INC.
8405 HELGERMAN COURT GAITHERSBURG, MD 20877-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Diagnosing DNA Mutations Using Mismatch Repair Enzymes
Agency: HHS
Contract: 2R44CA083441-02A1
Award Amount: $0.00
 

Abstract:

DESCRIPTION (provided by applicant): Trevigen/TreviMed has developed the Mutation Identification DNA Analysis System (MIDAS TM) that relies on DNA mismatch repair enzymes (MREs) for the detection and identification of known point mutations and pathogen DNA without the need for PCR amplification. The purpose of the research outlined in this Phase II proposal is to make MIDAS a robust and commercially viable clinical diagnostic product. To this end we propose the following specific aims: 1) increase the turnover rates of the MREs; 2) design MIDAS probes that incorporate donor/acceptor fluorophores which interact through fluorescence resonance energy transfer (FRET); and 3) optimize and clinically validate FRET-MIDAS reagents for the detection of high-risk oncogenic human papilloma virus (HPV) and the Coagulation factor V Leiden mutation, which are associated with cervical cancer and thrombophilia, respectively. Phase II support will lead to the development of a multiplexed, high-throughput, robust platform for detection of these proposed targets. We project that FRET-MIDAS will be able to accomplish all of this at a cost of $40 to $55 per test.

Principal Investigator:

Leonard S. Bazar
3012162800
LBAZAR@TREVIGEN.COM

Business Contact:

Michael Elliott
3012162800
MELLIOTT@TREVIGEN.COM
Small Business Information at Submission:

TREVIGEN, INC.
TREVIGEN, INC. 8405 HELGERMAN CT GAITHERSBURG, MD 20877

EIN/Tax ID: 521797207
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No