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Real Time Monitoring of Signaling Pathways in Biological Cells

Award Information

Department of Defense
Defense Advanced Research Projects Agency
Award ID:
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
UES, Inc.
4401 Dayton-Xenia Road Dayton, OH 45432-1894
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Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
Phase 2
Fiscal Year: 2005
Title: Real Time Monitoring of Signaling Pathways in Biological Cells
Agency / Branch: DOD / DARPA
Contract: W31P4Q-05-C-0227
Award Amount: $799,968.00


Cell function and survival are dictated by the transduction of the appropriate signaling pathways within the cell. These signaling pathways regulate nearly every aspect of cellular function from growth and proliferation, to progammed cell death, and sensing the surrounding environment. Until recently, investigation of cellular pathways has relied on destructive or after-the-fact techniques, including probing cell lysate for changes after a particular agonist has been added to the cell culture, or inferring protein activity in a pathway based on downstream events. We propose to investigate, in real time and in vivo, cellular pathways with a nanoprobe that can release or capture an enzyme or protein on demand. This probe is based on TiO2 nanoparticles, which are photoexcitable in the UV-range, and are attached to a molecular wire, through which this excitation can be harnessed. With modifications to this nanoprobe, it should be possible also to regulate gene transcription and use the nanoprobe in a biosensor platform to monitor the environment or solutions (including biologically-based solutions) for chemical or biological weapons.

Principal Investigator:

Melanie Tomczak
Research Scientist

Business Contact:

Francis F. Williams, Jr.
Contracts Manager
Small Business Information at Submission:

4401 Dayton-Xenia Road Dayton, OH 45432

EIN/Tax ID: 310797776
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No