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INTENSITY ENCODED DNA SEQUENCING

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16663
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16663
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
United States Biochemical Corp
26111 Miles Road Cleveland, OH 44128
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: INTENSITY ENCODED DNA SEQUENCING
Agency: HHS
Contract: N/A
Award Amount: $44,667.00
 

Abstract:

DNA SEQUENCING TYPICALLY REQUIRES FOUR PARALLEL REACTIONS RUN ON FOUR PARALLEL LANES OF AN ELECTROPHORESIS GEL. TOGETHER WITH GEL INTERPRETATION, THIS REPRESENTS MOST OF THE LABOR ONCE THE TEMPLATE DNA IS PREPARED. WE WILL DEVELOP A SIMPLIFIED DNA SEQUENCING METHOD USING A SINGLE REACTION AND A SINGLE LANE OF A GEL. THE SEQUENCE INFORMATION WILL BE ENCODED IN THE INTENSITIES OF THE BANDS. THIS INTENSITY-ENCODED SEQUENCING (IES) METHOD TAKES ADVANTAGE OF THREE RECENT ADVANCES CURRENTLY BEING MARKETED AND DEVELOPED AT UNITED STATES BIOCHEMICAL CORPORATION (USB). ONE IS SEQUENCE VERSION 2.0, A PATENTED ENZYME WHICH GENERATES EXCEPTIONAL SEQUENCING RESULTS. THE SECOND IS THE USE OF MN2+ AND INORGANIC PYROPHOSPHATASE WITH SEQUENASE. THESE MAKE CHAIN TERMINATION SEQUENCE-INDEPENDENT, GIVING UNIFORM BAND INTENSITIES. THUS, ALL FOUR DIDEOXY-NUCLEOTIDES CAN BE INCLUDED AT DIFFERENT CONCENTRATIONS IN A SINGLE REACTION; BAND INTENSITY DEPENDS ON THE CONCENTRATION, SO THE NUCLEOTIDE CAN BE DETERMINED FROM THE BAND INTENSITY. FINALLY, THE GELS ARE READ USING AN INEXPENSIVE PRECISION OPTICAL SCANNER TO BE INTRODUCED BY USB IN EARLY 1991. IES IS SIMPLE AND INEXPENSIVE ENOUGH TO BE RUN IN ANY LABORATORY THAT DOES SEQUENCING. COMMERCIAL PRODUCTS WILL INCLUDE A REAGENT KIT FOR RUNNING IES REACTIONS AND SOFTWARE TO INTERPRET GELS.

Principal Investigator:

Fuller, Carl W
Principal Investigator
2167655000

Business Contact:

1 r43 hg00191-01a1
Small Business Information at Submission:

United States Biochemical Corp
26111 Miles Road Cleveland, OH 44128

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No