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PRODUCTION OF HIV GP-120 PROTEIN

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11450
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11450
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Verax Corp
Etna Rd Hc 61 Box 6 Lebanon, NH 03766
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: PRODUCTION OF HIV GP-120 PROTEIN
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE TERMINAL NATURE OF HIV INFECTIONS HAS PROMPTED DEMANDS FOR LARGE QUANTITIES OF HIV PROTEINS FOR RESEARCH RELATED TO UNDERSTANDING THE VIRUS AND DEVELOPING THERAPEUTIC TREATMENTS. VERAX CORPORATION HAS DEVELOPED A UNIQUE SYSTEMFOR THE MASS CULTURE OF MAMMALIAN CELLS IN A CONTINUOUS FLUIDIZED BED SYSTEM. THE HEART OF THE TECHNOLOGY IS THE MICROSPHERE, A COLLAGEN-BASED MICROCARRIER THAT PERMITS CELL POPULATION DENSITIES IN EXCESS OF 10(8) CELLS/ML. THIS SYSTEM HAS ALREADY BEEN SUCCESSFULLY USED FOR THE LARGE-SCALE PRODUCTION OF SEVERAL PROTEIN THERAPEUTICS. THE GOAL OF THIS RESEARCH IS TWOFOLD. FIRST, IT IS PLANNED TO INVESTIGATE THE USE OF MICROSPHERES AS A SUBSTRATE FOR THE GROWTH AND PRODUCTION OF THE HIV-1 GP-120 PROTEIN BY A GENETICALLY ENGINEERED HELA CELL. THE GOAL OF THIS PORTION OF THE PROJECT IS TO DETERMINE THE EFFICACY OF USING THIS SYSTEM FOR GP-120 PRODUCTION AND TO OPTIMIZE THE CHARACTERISTICS OF THE MATRIX TO PERMIT MAXIMUM CELL DENSITYAND PRODUCTIVITY. SECOND, A CONTINUOUS CULTURE MICROREACTORWILL BE DEVELOPED FOR USE WITH MICROSPHERES. THE DEVELOPMENT OF SUCH A SYSTEM IS ESSENTIAL TO CONDUCTING THIS RESEARCH BECAUSE OF THE INADEQUACY OF OTHER SMALL-SCALEBIOREACTORS FOR CONDUCTING RESEARCH ON MICROSPHERES. IT MAYALSO PERMIT INEXPENSIVE LABORATORY-SCALE PRODUCTION OF THE GP-120 AND OTHER HIV PROTEINS.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Verax Corp
Etna Rd Hc 61 Box 6 Lebanon, NH 03766

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No