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HPLC INTERFACE FOR ELECTROSPRAY MASS SPECTROMETRY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13908
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13908
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Vestec Corpon
9299 Kirby Dr Houston, TX 77054
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: HPLC INTERFACE FOR ELECTROSPRAY MASS SPECTROMETRY
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE GOAL OF THIS RESEARCH IS TO DEVELOP AND ADAPT VESTEC CORPORATION'S "UNIVERSAL INTERFACE" TO COUPLE CONVENTIONAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TO ELECTROSPRAY MASS SPECTROMETRY. THE INTERFACE, WHICH HAS BEEN SUCCESSFULLY APPLIED TO COUPLING REVERSED-PHASE HPLC AT FLOW RATES AS HIGH AS 1.5 ML/MIN OF AQUEOUS EFFLUENT TO ELECTRON IMPACT MASS SPECTROMETRY, PRODUCES AN AEROSOL OF DROPLETS CONTAINING THE SAMPLE AND ALLOWS REMOVAL OF AS MUCHSOLVENT AS DESIRED WITHOUT LOSING THE SAMPLE OR DESTROYING THE CHROMATOGRAPHIC FIDELITY. ELECTROSPRAY MASS SPECTROMETRY IS AN EXCITING NEW TECHNIQUE WHEREBY MOLECULAR-WEIGHT INFORMATION CAN BE OBTAINED ON PROTEINS ANDOTHER BIOPOLYMERS WITH NO APPARENT LIMIT ON SIZE. THIS PROJECT WILL DEVELOP A TECHNIQUE FOR CHARGING THE DROPLETS EMERGING FROM THE UNIVERSAL INTERFACE AND TRANSMITTING THE RESULTING CHARGED DROPLETS AND/OR MULTIPLY-CHARGED MOLECULAR IONS TO A QUADRUPOLE MASS SPECTROMETER. IN THIS WAY, SPECTRA ON PROTEINS AND OTHER LARGE BIOMOLECULES WILL BE OBTAINED SIMILAR TO THOSE ALREADYDEMONSTRATED FOR ELECTROSPRAY, BUT WITHOUT THE RESTRICTIONS ON LIQUID FLOW AND COMPOSITION INHERENT IN THE CONVENTIONAL ELECTROSPRAY TECHNIQUE. THE ULTIMATE GOAL IS AN INSTRUMENT FOR DETERMINING THE MOLECULAR WEIGHTS ON SUBPICOMOLAR COMPONENTS OF COMPLEX BIOLOGICAL EXTRACTS ONLINE WITH HPLC SEPARATION.

Principal Investigator:

Marvin L Vestal
7137969677

Business Contact:

Small Business Information at Submission:

Vestec Corp
9299 Kirby Dr Houston, TX 77054

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No