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A Versatile Standard for Quantitative Light Microscopy

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66478
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
GM062671
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
VIRGINIA TECHNOLOGIES, INC.
660 Hunters Place CHARLOTTESVILLE, VA 22911-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2003
Title: A Versatile Standard for Quantitative Light Microscopy
Agency: HHS
Contract: 2R44GM062671-02
Award Amount: $510,129.00
 

Abstract:

DESCRIPTION (provided by applicant): Quantitative light microscopy is a valuable technique for studies of cellular function. New data concerning interactions of intracellular proteins, ion concentrations, membrane potential and additional forms of cellular activity are obtained from quantitative measurement of light from exogenous and endogenous optical probes. The accuracy of these measurements relies heavily on well calibrated and characterized microscope light detection systems. This proposal describes the commercial development of three instruments to benefit quantitative microscopy. The first instrument, Benchmark TM, is a versatile standard for quantitative light microscopy. A reliable and easily used high precision standard is not commercially available and would greatly facilitate reproducible measurements. Technologies currently used as light standards for microscopy present some practical difficulties. The calibrated light output of these devices is affected by illuminator lamp noise and aging. The light output of the electronic standard described in this proposal is independent of the microscope's illuminator output. Benchmark TM provides repeatable fiat-field illumination at multiple user selectable wavelengths and illumination levels from a single aperture. The second instrument, ilium TM can be used to monitor and record the intensity level of the microscope's illuminator. The third instrument, RatioLite TM, will provide dual wavelength emission for ratiometric recording of fluorescent dyes. These instruments are uniquely versatile, having operating modes unavailable in any competing product. This technology represents a significant technical innovation in the field of light microscopy.

Principal Investigator:

Robert A. Ross
4349702200
ROSS@VATECHNOLOGIES.COM

Business Contact:

Ross Robert
8049702200
ROSS@VATECHNOLOGIES.COM
Small Business Information at Submission:

VIRGINIA TECHNOLOGIES, INC.
VIRGINIA TECHNOLOGIES, INC. 2015 IVY RD, STE 423 CHARLOTTESVILLE, VA 22903

EIN/Tax ID: 541785431
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No