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Parallel Microfluidic System for High Throughput Label Free Cytokine Analysis

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
88810
Program Year/Program:
2008 / SBIR
Agency Tracking Number:
AI081533
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Wasatch Microfluidics, LLC
4909 BROWN VILLA COVE SALT LAKE CITY, UT 84123-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2008
Title: Parallel Microfluidic System for High Throughput Label Free Cytokine Analysis
Agency: HHS
Contract: 1R43AI081533-01
Award Amount: $199,776.00
 

Abstract:

DESCRIPTION (provided by applicant): The ultimate goal of this project is to develop a multiplex label free platform for high- throughput cytokine screening of patient samples. Though there are benefits to the current suspension phase multiplex cytokine a nalysis tools (Luminex), the technology suffers from a number of drawbacks including reagent variability, high reagent costs, and long run times. The development of a high throughput label free system will improve the throughput and reproducibility of cyto kine analyses. In addition, cost savings will be realized through reductions in development costs and the removal of labeling reagents. Accordingly, Wasatch Microfluidics will team with the Baylor Institute of Immunological Research (BIIR) to adapt Wasatch 's continuous flow spotting technologies to create a flow cell array directly integrated with a commercial biosensor array platform. Currently, flow cell technology is the limiting factor in the development of high throughput label free sensing technologie s. Modification of Wasatch Microfluidics Continuous Flow MicrospotterTM into a highly parallel flow cell should begin to eliminate this bottleneck and provide a template for even more highly parallel systems. Preliminary work suggests that a flow cell arra y can convert mediocre SPR imaging instruments into highly competitive protein analysis instruments comparable to state-of-the-art SPR instruments with meager throughput. Once the flow cell is completed and integrated with the commercial biosensor platform at the BIIR, validation testing will be conducted to demonstrate cytokine discrimination and quantitative measurement of cytokine levels in a high throughput multiplex label free format.

Principal Investigator:

Business Contact:


bruce@microfl.com
Small Business Information at Submission:

WASATCH MICROFLUIDICS
4909 BROWN VILLA COVE SALT LAKE CITY, UT 84123

EIN/Tax ID: 830413045
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No