USA flag logo/image

An Official Website of the United States Government

Rapid Detection of Listeria Monocytogenes

Award Information

Agency:
Department of Agriculture
Branch:
N/A
Award ID:
43639
Program Year/Program:
1999 / SBIR
Agency Tracking Number:
1999-00307
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Xtrana, Inc.
590 Burbank St., Ste. 205 Broomfield, CO 80020
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1999
Title: Rapid Detection of Listeria Monocytogenes
Agency: USDA
Contract: 99-33610-7516
Award Amount: $65,000.00
 

Abstract:

Not Available Listeria monocytogenes is a widely distributed food pathogen that has recently been associated with a number of food recalls and outbreaks of illness. Xtrana, Inc. is developing a low complexity genetic- based assay for sensitive and specific detection of L. monocytogenes. The successful development and validation of our assay will provide for confirmed detection of Listeria monocytogenes with vastly improved turnaround times. We anticipate that following minimal enrichment times, the assay will be run directly without the usual selective culture and subculture. In Phase I feasibility of extraction of RNA, capture onto a solid phase, isothermal amplification, and visual result detection was demonstrated. We are now ready to proceed with more rigorous sensitivity and specificity confirmation, interface with all of the food types required for AOAC approval, and confirmation that the assay will be specific to only viable Listeria monocytogenes.

Principal Investigator:

Jeffrey M. Marmaro
3033632465
jmarmaro@mi-mail.com

Business Contact:

John H. Wheeler
President and CEO
3033632445
jwheeler@mi-mail.com
Small Business Information at Submission:

Xtrana, Inc.
717 Yosemite Circle Denver, CO 80230

EIN/Tax ID: 841450164
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No